Bacterial Conjugation: Definition & Protocol - Video

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Bacterial Conjugation- Definition, Principle, Process, Examples

Bacterial Conjugation- Definition, Principle, Process, Examples submitted by broneps to EverythingScience [link] [comments]

AP Bio Guide (Units 8 in comments)

AP Bio Guide (Units 8 in comments)

1) Chemistry of Life

Content

  • Transpiration
    • Hydrogen bonds pull water up like string and leave through stoma
    • Stomata: leaf pores that allow gas exchange, most are on bottom side of leaf
    • Xylem: tube-shaped, nonlining, vascular system, carries water from roots to rest of plant
    • Epidermis: outer layer, protects plant
    • Phloem: transports food
    • Parenchyma: stores food
    • Transpiration: evaporation of water from leaves
    • Adhesion: polar water molecules adhere to polar surfaces (sides of xylem)
    • Cohesion: polar water molecules adhere to each other
    • Guard cells: cells surrounding stoma, regulate transpiration through opening and closing stoma
    • Turgid vs flaccid guard cells
      • Turgid swell caused by potassium ions, water potential decreases, water enters vacuoles of guard cells
      • Swelling of guard cells open stomata
    • High light levels, high levels of water, low temperature, low CO2 causes opening of stomata
    • Water potential: transport of water in plant governed by differences in water potential
      • Affected by solute concentration and environmental conditions
    • High water potential (high free energy and more water) travels to low water potential
    • Hydrophilic = attracts water, hydrophobic = repels water
  • Water and its Properties
    • Polar molecule due to positive hydrogen and negative oxygen regions
    • Negative oxygen of one molecule to positive hydrogen of another water molecule forms a hydrogen bond, which are weak individually but strong together
    • Important physical properties of water:
      • Cohesion and adhesion: cohesion creates surface tension and they both allow for transpiration
      • High specific heat: enables water to absorb and lose heat slowly
      • High heat of vaporization: allows much of it to remain liquid
      • Nearly universal polar solvent: dissolves a lot of stuff
      • Flotation of ice: insulates, transportation
  • Biological Macromolecules
    • Polymer: long molecule consisting of many similar building blocks linked by covalent bonds
    • Monomer: building block of a polymer
    • ATP - adenosine triphosphate, energy carrier that uses bonds between phosphates to store energy
      • Similar in structure to a ribonucleotide
    • Four Types
      • Carbohydrates
      • Lipids
      • Proteins
      • Nucleic Acids
https://preview.redd.it/xp12oli61w451.png?width=1098&format=png&auto=webp&s=cc897738989258c67bcc760ba040e2cee8f7875c
  • Functional groups
    • Hydroxyl - carbs, alcohols - OH-, O-
    • Amino - proteins - NH2, NH3+
    • Carboxyl - weak acids - COOH, COO-
    • Sulfhydryl - proteins - SH
    • Phosphatic - salts, strong acids - PO
  • Directionality:
    • ex: glucose alpha and beta
    • ex: DNA and RNA 5’ and 3’ ends
  • Identification of Macromolecules
https://preview.redd.it/cb3oau2j1w451.png?width=1089&format=png&auto=webp&s=409e26f32c9996a3649bad81d17ed72769955ce9

Calculations

  • Number of bonds
    • # of molecules - 1
    • i.e. 20 glucose molecules linked together would have 19 bonds
  • Molecular formula
    • # of molecules * molecular formula - number of bonds * H20 (from hydrolysis)
    • i.e. when you bond 5 glucose molecules together you have to subtract 4H2O
  • pH/pOH
    • -log[H+] = pH
    • -log[OH-] = pOH
    • pH + pOH = 14
  • Leaf surface area
    • i.e. using graph paper to find surface area
  • Transpiration rate
    • Amount of water used / surface area / time

Labs

  • Transpiration Lab
    • Basically you take this potometer which measures the amount of water that gets sucked up by a plant that you have and you expose the plant to different environmental conditions (light, humidity, temperature) and see how fast the water gets transpired
    • Random stuff to know:
      • It’s hard to get it to work properly
      • A tight seal of vaseline keeps everything tidy and prevents water from evaporating straight from the tube, also allows for plant to suck properly
      • Water travels from high water potential to low water potential

2) Cell Structure & Function

Content

  • Cellular Components
    • Many membrane-bound organelles evolved from once free prokaryotes via endosymbiosis, such as mitochondria (individual DNA)
    • Compartmentalization allows for better SA:V ratio and helps regulate cellular processes
    • Cytoplasm: thick solution in each cell containing water, salts, proteins, etc; everything - nucleus
      • Cytoplasmic streaming: moving all the organelles around to give them nutrients, speeds up reactions
    • Cytosol: liquid of the cytoplasm (mostly water)
    • Plasma Membrane: separates inside of cell from extracellular space, controls what passes through amphipathic area (selectively permeable)
      • Fluid-Mosaic model: phospholipid bilayer + embedded proteins
      • Aquaporin: hole in membrane that allows water through
    • Cell Wall: rigid polysaccharide layer outside of plasma membrane in plants/fungi/bacteria
      • Bacteria have peptidoglycan, fungi have chitin, and plants have cellulose and lignin
      • Turgor pressure pushes the membrane against the wall
    • Nucleus: contains genetic information
      • Has a double membrane called the nuclear envelope with pores
    • Nucleolus: in nucleus, produces ribosomes
    • Chromosomes: contain DNA
    • Centrioles: tubulin thing that makes up centrosome in the middle of a chromosome
    • Smooth Endoplasmic Reticulum: storage of proteins and lipids
    • Rough Endoplasmic Reticulum: synthesizes and packages proteins
    • Chloroplasts: photosynthetic, sunlight transferred into chemical energy and sugars
      • More on this in photosynthesis
    • Vacuoles: storage, waste breakdown, hydrolysis of macromolecules, plant growth
    • Plasmodesmata: channels through cell walls that connect adjacent cells
    • Golgi Apparatus: extracellular transport
    • Lysosome: degradation and waste management
      • Mutations in the lysosome cause the cell to swell with unwanted molecules and the cell will slow down or kill itself
    • Mitochondria: powerhouse of the cell
      • Mutations in the mitochondria cause a lack of deficiency of energy in the cell leading to an inhibition of cell growth
    • Vesicles: transport of intracellular materials
    • Microtubules: tubulin, stiff, mitosis, cell transport, motor proteins
    • Microfilaments: actin, flexible, cell movement
    • Flagella: one big swim time
    • Cilia: many small swim time
    • Peroxisomes: bunch of enzymes in a package that degrade H202 with catalase
    • Ribosomes: protein synthesis
    • Microvilli: projections that increase cell surface area like tiny feetsies
      • In the intestine, for example, microvilli allow more SA to absorb nutrients
    • Cytoskeleton: hold cell shape
  • Cellular Transport
    • Passive transport: diffusion
      • Cell membranes selectively permeable (large and charged repelled)
      • Tonicity: osmotic (water) pressure gradient
    • Cells are small to optimize surface area to volume ratio, improving diffusion
    • Primary active transport: ATP directly utilized to transport
    • Secondary active transport: something is transported using energy captured from movement of other substance flowing down the concentration gradient
    • Endocytosis: large particles enter a cell by membrane engulfment
      • Phagocytosis: “cell eating”, uses pseudopodia around solids and packages it within a membrane
      • Pinocytosis: “cell drinking”, consumes droplets of extracellular fluid
      • Receptor-mediated endocytosis: type of pinocytosis for bulk quantities of specific substances
    • Exocytosis: internal vesicles fuse with the plasma membrane and secrete large molecules out of the cell
    • Ion channels and the sodium potassium pump
      • Ion channel: facilitated diffusion channel that allows specific molecules through
      • Sodium potassium pump: uses charged ions (sodium and potassium)
    • Membrane potential: voltage across a membrane
    • Electrogenic pump: transport protein that generates voltage across a membrane
    • Proton pump: transports protons out of the cell (plants/fungi/bacteria)
    • Cotransport: single ATP-powered pump transports a specific solute that can drive the active transport of several other solutes
    • Bulk flow: one-way movement of fluids brought about by pressure
    • Dialysis: diffusion of solutes across a selective membrane
  • Cellular Components Expanded: The Endomembrane System
    • Nucleus + Rough ER + Golgi Bodies
      • Membrane and secretory proteins are synthesized in the rough endoplasmic reticulum, vesicles with the integral protein fuse with the cis face of the Golgi apparatus, modified in Golgi, exits as an integral membrane protein of the vesicles that bud from the Golgi’s trans face, protein becomes an integral portion of that cell membrane

Calculations

  • Surface area to volume ratio of a shape (usually a cube)
  • U-Shaped Tube (where is the water traveling)
    • Solution in u-shaped tube separated by semi-permeable membrane
    • find average of solute (that is able to move across semi permeable membrane)
    • add up total molar concentration on both sides
    • water travels where concentration is higher
  • Water Potential = Pressure Potential + Solute Potential
    • Solute Potential = -iCRT
      • i = # of particles the molecule will make in water
      • C = molar concentration
      • R = pressure constant (0.0831)
      • T = temperature in kelvin

Labs

  • Diffusion and Osmosis
    • Testing the concentration of a solution with known solutions
    • Dialysis bag
      • Semipermeable bag that allows the water to pass through but not the solute
    • Potato core
      • Has a bunch of solutes inside

Relevant Experiments

  • Lynne Margolis: endosymbiotic theory (mitochondria lady)
  • Chargaff: measured A/G/T/C in everything (used UV chromatography)
  • Franklin + Watson and Crick: discovered structure of DNA; Franklin helped with x ray chromatography

3) Cellular Energetics

Content

  • Reactions and Thermodynamics
    • Baseline: used to establish standard for chemical reaction
    • Catalyst: speeds up a reaction (enzymes are biological catalysts)
    • Exergonic: energy is released
    • Endergonic: energy is consumed
    • Coupled reactions: energy lost/released from exergonic reaction is used in endergonic one
    • Laws of Thermodynamics:
      • First Law: energy cannot be created nor destroyed, and the sum of energy in the universe is constant
      • Second Law: energy transfer leads to less organization (greater entropy)
      • Third Law: the disorder (entropy) approaches a constant value as the temperature approaches 0
    • Cellular processes that release energy may be coupled with other cellular processes
    • Loss of energy flow means death
    • Energy related pathways in biological systems are sequential to allow for a more controlled/efficient transfer of energy (product of one metabolic pathway is reactant for another)
    • Bioenergetics: study of how energy is transferred between living things
    • Fuel + 02 = CO2 + H20
      • Combustion, Photosynthesis, Cellular Respiration (with slight differences in energy)
  • Enzymes
    • Speed up chemical processes by lowering activation energy
    • Structure determines function
    • Active sites are selective
    • Enzymes are typically tertiary- or quaternary-level proteins
    • Catabolic: break down / proteases and are exergonic
    • Anabolic: build up and are endergonic
    • Enzymes do not change energy levels
    • Substrate: targeted molecules in enzymatic
    • Many enzymes named by ending substrate in “-ase”
    • Enzymes form temporary substrate-enzyme complexes
    • Enzymes remain unaffected by the reaction they catalyze
    • Enzymes can’t change a reaction or make other reactions occur
    • Induced fit: enzyme has to change its shape slightly to accommodate the substrate
    • Cofactor: factor that help enzymes catalyze reactions (org or inorg)
      • Examples: temp, pH, relative ratio of enzyme and substrate
      • Organic cofactors are called coenzymes
    • Denaturation: enzymes damaged by heat or pH
    • Regulation: protein’s function at one site is affected by the binding of regulatory molecule to a separate site
    • Enzymes enable cells to achieve dynamic metabolism - undergo multiple metabolic processes at once
    • Cannot make an endergonic reaction exergonic
    • Steps to substrates becoming products
      • Substrates enters active site, enzyme changes shape
      • Substrates held in active site by weak interactions (i.e. hydrogen bonds)
      • Substrates converted to product
      • Product released
      • Active site available for more substrate
    • Rate of enzymatic reaction increases with temperature but too hot means denaturation
    • Inhibitors fill the active site of enzymes
      • Some are permanent, some are temporary
      • Competitive: block substrates from their active sites
      • Non competitive (allosteric): bind to different part of enzyme, changing the shape of the active site
    • Allosteric regulation: regulatory molecules interact with enzymes to stimulate or inhibit activity
    • Enzyme denaturation can be reversible
  • Cellular Respiration
    • Steps
      • Glycolysis
      • Acetyl co-A reactions
      • Krebs / citric acid cycle
      • Oxidative phosphorylation
    • Brown fat: cells use less efficient energy production method to make heat
    • Hemoglobin (transport, fetal oxygen affinity > maternal) and myoglobin (stores oxygen)
  • Photosynthesis
    • 6CO2 + 6H20 + Light = C6H12O6 + 6O2
    • Absorption vs action spectrum (broader, cumulative, overall rate of photosynthesis)
    • Components
      • Chloroplast
      • Mesophyll: interior leaf tissue that contains chloroplasts
      • Pigment: substance that absorbs light
    • Steps
      • Light-Dependent Reaction
      • Light-Independent (Dark) Reaction (Calvin Cycle)
  • Anaerobic Respiration (Fermentation)
    • Glycolysis yields 2ATP + 2NADH + 2 Pyruvate
    • 2NADH + 2 Pyruvate yields ethanol and lactate
    • Regenerates NAD+

Calculations

  • Calculate products of photosynthesis & cellular respiration

Labs

  • Enzyme Lab
    • Peroxidase breaks down peroxides which yields oxygen gas, quantity measured with a dye
    • Changing variables (i.e. temperature) yields different amounts of oxygen
  • Photosynthesis Lab
    • Vacuum in a syringe pulls the oxygen out of leaf disks, no oxygen causes them to sink in bicarbonate solution, bicarbonate is added to give the disks a carbon source for photosynthesis which occurs at different rates under different conditions, making the disks buoyant
  • Cellular Respiration Lab
    • Use a respirometer to measure the consumption of oxygen (submerge it in water)
    • You put cricket/animal in the box that will perform cellular respiration
    • You put KOH in the box with cricket to absorb the carbon dioxide (product of cellular respiration)-- it will form a solid and not impact your results

Relevant Experiments

  • Engelmann
    • Absorption spectra dude with aerobic bacteria

4) Cell Communication & Cell Cycle

Content

  • Cell Signalling
    • Quorum sensing: chemical signaling between bacteria
      • See Bonnie Bassler video
    • Taxis/Kinesis: movement of an organism in response to a stimulus (chemotaxis is response to chemical)
    • Ligand: signalling molecule
    • Receptor: ligands bind to elicit a response
    • Hydrophobic: cholesterol and other such molecules can diffuse across the plasma membrane
    • Hydrophilic: ligand-gated ion channels, catalytic receptors, G-protein receptor
  • Signal Transduction
    • Process by which an extracellular signal is transmitted to inside of cell
    • Pathway components
      • Signal/Ligand
      • Receptor protein
      • Relay molecules: second messengers and the phosphorylation cascade
      • DNA response
    • Proteins in signal transduction can cause cancer if activated too much (tumor)
      • RAS: second messenger for growth factor-- suppressed by p53 gene (p53 is protein made by gene) if it gets too much
    • Response types
      • Gene expression changes
      • Cell function
      • Alter phenotype
      • Apoptosis- programmed cell death
      • Cell growth
      • Secretion of various molecules
    • Mutations in proteins can cause effects downstream
    • Pathways are similar and many bacteria emit the same chemical within pathways, evolution!
  • Feedback
    • Positive feedback amplifies responses
      • Onset of childbirth, lactation, fruit ripening
    • Negative feedback regulates response
      • Blood sugar (insulin goes down when glucagon goes up), body temperature
  • Cell cycle
    • Caused by reproduction, growth, and tissue renewal
    • Checkpoint: control point that triggers/coordinates events in cell cycle
    • Mitotic spindle: microtubules and associated proteins
      • Cytoskeleton partially disassembles to provide the material to make the spindle
      • Elongates with tubulin
      • Shortens by dropping subunits
      • Aster: radial array of short microtubules
      • Kinetochores on centrosome help microtubules to attach to chromosomes
    • IPMAT: interphase, prophase, metaphase, anaphase, telophase
      • PMAT is mitotic cycle
    • Steps
      • Interphase
      • Mitosis
      • Cytokinesis
    • Checkpoints
      • 3 major ones during cell cycle:
      • cyclin-cdk-mpf: cyclin dependent kinase mitosis promoting factor
      • Anchorage dependence: attached, very important aspect to cancer
      • Density dependence: grow to a certain size, can’t hurt organs
      • Genes can suppress tumors
    • G0 phase is when cells don’t grow at all (nerve, muscle, and liver cells)

Calculations

Relevant Experiments

  • Sutherland
    • Broke apart liver cells and realized the significance of the signal transduction pathway, as the membrane and the cytoplasm can’t activate glycogen phosphorylase by themselves

5) Heredity

Content

  • Types of reproduction
    • Sexual: two parents, mitosis/meiosis, genetic variation/diversity (and thus higher likelihood of survival in a changing environment)
    • Asexual: doesn’t require mate, rapid, almost genetically identitical (mutations)
      • Binary fission (bacteria)
      • Budding (yeast cells)
      • Fragmentation (plants and sponges)
      • Regeneration (starfish, newts, etc.)
  • Meiosis
    • One diploid parent cell undergoes two rounds of cell division to produce up to four haploid genetically varied cells
    • n = 23 in humans, where n is the number of unique chromosomes
    • Meiosis I
      • Prophase: synapsis (two chromosome sets come together to form tetrad), chromosomes line up with homologs, crossing over
      • Metaphase: tetrads line up at metaphase plate, random alignment
      • Anaphase: tetrad separation, formation at opposite poles, homologs separate with their centromeres intact
      • Telophase: nuclear membrane forms, two haploid daughter cells form
    • Meiosis II
      • Prophase: chromosomes condense
      • Metaphase: chromosomes line up single file, not pairs, on the metaphase plate
      • Anaphase: chromosomes split at centromere
      • Telophase: nuclear membrane forms and 4 total haploid cells are produced
    • Genetic variation
      • Crossing over: homologous chromosomes swap genetic material
      • Independent assortment: homologous chromosomes line up randomly
      • Random fertilization: random sperm and random egg interact
    • Gametogenesis
      • Spermatogenesis: sperm production
      • Oogenesis: egg cells production (¼ of them degenerate)
  • Fundamentals of Heredity
    • Traits: expressed characteristics
    • Gene: “chunk” of DNA that codes for a specific trait
    • Homologous chromosomes: two copies of a gene
    • Alleles: copies of chromosome may differ bc of crossing over
    • Homozygous/Heterozygous: identical/different
    • Phenotype: physical representation of genotype
    • Generations
      • Parent or P1
      • Filial or F1
      • F2
    • Law of dominance: one trait masks the other one
      • Complete: one trait completely covers the other one
      • Incomplete: traits are both expressed
      • Codominance: traits combine
    • Law of segregation (Mendel): each gamete gets one copy of a gene
    • Law of independent assortment (Mendel): traits segregate independently from one another
    • Locus: location of gene on chromosome
    • Linked genes: located on the same chromosome, loci less than 50 cM apart
    • Gene maps and linkage maps
    • Nondisjunction: inability of chromosomes to separate (ex down syndrome)
    • Polygenic: many genes influence one phenotype
    • Pleiotropic: one gene influences many phenotypes
    • Epistasis: one gene affects another gene
    • Mitochondrial and chloroplast DNA is inherited maternally
  • Diseases/Disorders
    • Genetic:
      • Tay-Sachs: can’t break down specific lipid in brain
      • Sickle cell anemia: misshapen RBCs
      • Color blindness
      • Hemophilia: lack of clotting factors
    • Chromosomal:
      • Turner: only one X chromosome
      • Klinefelter: XXY chromosomes
      • Down syndrome (trisomy 21): nondisjunction
  • Crosses
    • Sex-linked stuff
    • Blood type
    • Barr bodies: in women, two X chromosomes; different chromosomes expressed in different parts of the body, thus creating two different phenotype expressions in different places

Calculations

  • Pedigree/Punnett Square
  • Recombination stuff
    • Recombination rate = # of recombinable offspring/ total offspring (times 100) units: map units

Relevant Experiments

  • Mendel

6) Gene Expression and Regulation

Content

  • DNA and RNA Structure
    • Prokaryotic organisms typically have circular chromosomes
    • Plasmids = extrachromosomal circular DNA molecules
    • Purines (G, A) are double-ringed while pyrimidines (C, T, U) have single ring
    • Types of RNA:
      • mRNA - (mature) messenger RNA (polypeptide production)
      • tRNA - transfer RNA (polypeptide production)
      • rRNA - ribosomal RNA (polypeptide production)
      • snRNA - small nuclear RNA (bound to snRNPs - small nuclear ribonucleoproteins)
      • miRNA - microRNA (regulatory)
  • DNA Replication
    • Steps:
      • Helicase opens up the DNA at the replication fork.
      • Single-strand binding proteins coat the DNA around the replication fork to prevent rewinding of the DNA.
      • Topoisomerase works at the region ahead of the replication fork to prevent supercoiling.
      • Primase synthesizes RNA primers complementary to the DNA strand.
      • DNA polymerase III extends the primers, adding on to the 3' end, to make the bulk of the new DNA.
      • RNA primers are removed and replaced with DNA by DNA polymerase I.
      • The gaps between DNA fragments are sealed by DNA ligase.
  • Protein Synthesis
    • 61 codons code for amino acids, 3 code as STOP - UAA, UAG, UGA - 64 total
    • Transcription Steps:
      • RNA polymerase binds to promoter (before gene) and separate the DNA strands
      • RNA polymerase fashions a complementary RNA strand from a DNA strand
      • Coding strand is same as RNA being made, template strand is complementary
      • Terminator on gene releases the RNA polymerase
    • RNA Processing Steps (Eukaryotes):
      • 5’ cap and 3’ (poly-A tail, poly A polymerase) tail is added to strand (guanyl transferase)
      • Splicing of the RNA occurs in which introns are removed and exons are added by spliceosome
      • Cap/tail adds stability, splicing makes the correct sequence (“gibberish”)
    • Translation Steps:
      • Initiation complex is the set up of a ribosome around the beginning of an mRNA fragment
      • tRNA binds to codon, amino acid is linked to other amino acid
      • mRNA is shifted over one codon (5’ to 3’)
      • Stop codon releases mRNA
  • Gene Expression
    • Translation of mRNA to a polypeptide occurs on ribosomes in the cytoplasm as well as rough ER
    • Translation of the mRNA occurs during transcription in prokaryotes
    • Genetic info in retroviruses is an exception to normal laws: RNA to DNA is possible with reverse transcriptase, which allows the virus to integrate into the host’s DNA
    • Regulatory sequences = stretches of DNA that interact with regulatory proteins to control transcription
    • Epigenetic changes can affect expression via mods of DNA or histones
    • Observable cell differentiation results from the expression of genes for tissue-specific proteins
    • Induction of transcription factors during dev results in gene expression
    • Prokaryotes: operons transcribed in a single mRNA molecule, inducible system
    • Eukaryotes: groups of genes may be influenced by the same transcription factors to coordinate expression
    • Promoters = DNA sequences that RNA polymerase can latch onto to initiate
    • Negative regulators inhibit gene expression by binding to DNA and blocking transcription
    • Acetylation (add acetyl groups)- more loosely wound/ less tightly coiled/compressed
    • Methylation of DNA (add methyl groups) - less transcription- more tightly wound
  • Mutation and Genetic Variation
    • Disruptions in genes (mutations) change phenotypes
    • Mutations can be +/-/neutral based on their effects that are conferred by the protein formed - environmental context
    • Errors in DNA replication or repair as well as external factors such as radiation or chemical exposure cause them
    • Mutations are the primary source of genetic variation
    • Horizontal acquisition in prokaryotes - transformation (uptake of naked DNA), transduction (viral DNA transmission), conjugation (cell-cell DNA transfer), and transposition (DNA moved within/between molecules) - increase variation
    • Related viruses can (re)combine genetic material in the same host cell
    • Types of mutations: frameshift, deletion, insertion
  • Genetic Engineering
    • Electrophoresis separates molecules by size and charge
    • PCR magnifies DNA fragments
    • Bacterial transformation introduces DNA into bacterial cells
  • Operons
    • Almost always prokaryotic
    • Promoter region has operator in it
    • Structural genes follow promoter
    • Terminator ends operon
    • Regulatory protein is active repressor
    • Active repressor can be inactivated
    • Enhancer: remote gene that require activators
    • RNAi: interference with miRNA
    • Anabolic pathways are normally on and catabolic pathways are normally off

Calculations

  • Transformation efficiency (colonies/DNA)
  • Numbers of base pairs (fragment lengths)
  • Cutting enzymes in a plasmid or something (finding the lengths of each section)

Labs

  • Gel Electrophoresis Lab
    • Phosphates in DNA make it negative (even though it’s an acid!), so it moves to positive terminal on the board
    • Smaller DNA is quicc, compare it to a standard to calculate approx. lengths
  • Bacterial Transformation Lab
    • Purpose of sugar: arabinose is a promoter which controls the GFP in transformed cells, turns it on, also green under UV
    • Purpose of flipping upside down: condensation forms but doesn’t drip down
    • Purpose of heat shock: increases bacterial uptake of foreign DNA
    • Plasmids have GFP (green fluorescent protein) and ampicillin resistance genes
    • Calcium solution puts holes in bacteria to allow for uptake of plasmids
  • PCR Lab
    • DNA + primers + nucleotides + DNA polymerase in a specialized PCR tube in a thermal cycler
    • Primers bind to DNA before it can repair itself, DNA polymerase binds to the primers and begins replication
    • After 30 cycles, there are billions of target sequences

Relevant Experiments

  • Avery: harmful + harmless bacteria in mice, experimented with proteins vs DNA of bacteria
  • Griffith: Avery’s w/o DNA vs protein
  • Hershey and Chase: radioactively labeled DNA and protein
  • Melson and Stahl: isotopic nitrogen in bacteria, looked for cons/semi/dispersive DNA
  • Beadle and Tatum: changed medium’s amino acid components to find that a metabolic pathway was responsible for turning specific proteins into other proteins, “one gene one enzyme”
  • Nirenberg: discovered codon table

7) Natural Selection

  • Scientific Theory: no refuting evidence (observation + experimentation), time, explain a brand/extensive range of phenomena
  • Theory of Natural Selection
    • Definition
      • Not all offspring (in a population) will survive
      • Variation among individuals in a population
      • Some variations were more favourable than others in a particular environment
      • Those with more favourable variations were more likely to survive and reproduce.
      • These favourable variations were passed on and increased in frequency over time.
  • Types of Selection:
    • Directional selection: one phenotype favored at one of the extremes of the normal distribution
      • ”Weeds out” one phenotype
      • Ony can happen if a favored allele is already present
    • Stabilizing Selection: Organisms within a population are eliminated with extreme traits
      • Favors “average” or medium traits
      • Ex. big head causes a difficult delivery; small had causes health deficits
    • Disruptive Selection: favors both extremes and selects against common traits
      • Ex. sexual selection (seems like directional but it’s not because it only affects one sex, if graph is only males then directional)
  • Competition for limited resources results in differential survival, favourable phenotypes are more likely to survive and produce more offspring, thus passing traits to subsequent generations.
    • Biotic and abiotic environments can be more or less stable/fluctuating, and this affects the rate and direction of evolution
      • Convergent evolution occurs when similar selective pressures result in similar phenotypic adaptations in different populations or species.
      • Divergent evolution: groups from common ancestor evolve, homology
      • Different genetic variations can be selected in each generation.
      • Environments change and apply selective pressures to populations.
    • Evolutionary fitness is measured by reproductive success.
    • Natural selection acts on phenotypic variations in populations.
      • Some phenotypic variations significantly increase or decrease the fitness of the organism in particular environments.
    • Through artificial selection, humans affect variation in other species.
      • Humans choose to cause artificial selection with specific traits, accidental selection caused by humans is not artificial
    • Random occurrences
      • Mutation
      • Genetic drift - change in existing allele frequency
      • Migration
    • Reduction of genetic variation within a given population can increase the differences between populations of the same species.
    • Conditions for a population or an allele to be in Hardy-Weinberg equilibrium are
      • Large population size
      • Absence of migration
      • No net mutations
      • Random mating
      • Absence of selection
    • Changes in allele frequencies provide evidence for the occurrence of evolution in a population.
    • Small populations are more susceptible to random environmental impact than large populations.
    • Gene flow: transference of genes/alleles between populations
  • Speciation: one species splits off into multiple species
    • Sympatric (living together i.e. disruption) Allopatric (physically separate, i.e. founder effect) Parapatric (habitats overlapping)
      • Polyploidy (autopolyploidy), sexual selection
    • Species: group of populations whose members can interbreed and produce healthy, fertile offspring but can’t breed with other species (ex. a horse and donkey can produce a mule but a mule is nonviable, so it doesn’t qualify)
      • Morphological definition: body shape and structural characteristics define a species
      • Ecological species definition: way populations interact with their environments define a species
      • Phylogenetic species definition: smallest group that shares a common ancestor is a species
    • Prezygotic barriers: barriers to reproduction before zygote is formed
      • Geographical error: two organisms are in different areas
      • Behavioural error (i.e. mating rituals aren’t the same)
      • Mechanical error: “the pieces don’t fit together”
      • Temporal error (i.e. one organism comes out at night while the other comes out in the day)
      • Zygotic/Gametic isolation: sperm and egg don’t physically meet
    • Postzygotic barriers: barriers to reproduction after zygote is formed
      • Hybrid viability: developmental errors of offspring
      • Hybrid fertility: organism is sterilized
      • Hybrid breakdown: offspring over generations aren’t healthy
    • Hybrid zone: region in which members of different species meet and mate
      • Reinforcement: hybrids less fit than parents, die off, strength prezygotic barriers
      • Fusion: two species may merge into one population
      • Stability: stable hybrid zones mean hybrids are more fit than parents, thus creating a stable population, but can be selected against in hybrid zones as well
    • Punctuated equilibria: long periods of no or little change evolutionarily punctuated by short periods of large change, gradualism is just slow evolution
    • Evidence of evolution
      • Paleontology (Fossils)
      • Comparative Anatomy
      • Embryology: embryos look the same as they grow
      • Biogeography: distribution of flora and fauna in the environment (pangea!)
      • Biochemical: DNA and proteins and stuff, also glycolysis
    • Phylogenetic trees
      • Monophyletic: common ancestor and all descendants
      • Polyphyletic: descendants with different ancestors
      • Paraphyletic: leaving specifies out of group
    • Out group: basal taxon, doesn’t have traits others do
    • Cline: graded variation within species (i.e. different stem heights based on altitude)
    • Anagenesis: one species turning into another species
    • Cladogenesis: one species turning into multiple species
    • Taxon: classification/grouping
    • Clade: group of species with common ancestor
    • Horizontal gene transfer: genes thrown between bacteria
    • Shared derived characters: unique to specific group
    • Shared primitive/ancestral characters: not unique to a specific group but is shared within group
  • Origins of life
    • Stages
      • Inorganic formation of organic monomers (miller-urey experiment)
      • Inorganic formation of organic polymers (catalytic surfaces like hot rock or sand)
      • Protobionts and compartmentalization (liposomes, micelles)
      • DNA evolution (RNA functions as enzyme)
    • Shared evolutionary characteristics across all domains
      • Membranes
      • Cell comm.
      • Gene to protein
      • DNA
      • Proteins
    • Extant = not extinct
    • Highly conserved genes = low rates of mutation in history due to criticalness (like electron transport chain)
    • Molecular clock: dating evolution using DNA evidence
    • Extinction causes niches for species to fill
    • Eukaryotes all have common ancestor (shown by membrane-bound organelles, linear chromosomes, and introns)

Calculations

  • Hardy-Weinberg
    • p + q = 1
    • p^2 + 2pq +q^2 = 1
  • Chi Squared

Labs

  • Artificial Selection Lab
    • Trichrome trait hairs
    • Anthocyanin for second trait (purple stems)
    • Function of the purple pigment?
    • Function of trichome hairs?
  • BLAST Lab
    • Putting nucleotides into a database outputs similar genes

Relevant Experiments

  • Darwin
  • Lamarck
  • Miller-Urey
    • Slapped some water, methane, ammonia, and hydrogen is some flasks and simulated early earth with heat and stuff and it made some amino acids.
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How to Manage Lactose Intolerance

Lactose intolerance is a condition in which people have symptoms due to the decreased ability to digest lactose, a sugar found in dairy products. Those affected vary in the amount of lactose they can tolerate before symptoms develop. Symptoms may include abdominal pain, bloating, diarrhea, gas, and nausea. These symptoms typically start thirty minutes to two hours after eating or drinking milk-based food. Severity typically depends on the amount a person eats or drinks. Lactose intolerance does not cause damage to the gastrointestinal tract.
How to Manage Lactose Intolerance – Causes, Symptoms and Treatment
Lactose intolerance is a prevalent and distressing condition that affects a surprisingly high percentage of adults. The U.S. Department of Health & Human Services approximates that about 65 percent of the human population has a reduced ability to digest lactose after infancy.
Lactose intolerance is not the same as a milk allergy and is more of a discomfort than a real over-reaction by the immune system, according to the FDA. Many people with lactose intolerance can even have small amounts of the offending foods/drinks without having symptoms.
What sort of signs of lactose intolerance may indicate that you have this common problem? Lactose intolerance symptoms typically include bloating, gas, diarrhea and other GI issues. Fortunately, by following a lactose intolerance diet and treatment plan, it’s possible to reduce (and in some cases even eliminate) the symptoms of lactose intolerance.

What Is Lactose Intolerance?

The definition of lactose intolerance, according to The National Institute of Diabetes and Digestive and Kidney Diseases, is “a condition in which you have digestive symptoms — such as bloating, diarrhea and gas — after you consume foods or drinks that contain lactose.”
Lactose is a sugar that is found in milk and dairy products. In order to digest this sugar properly, the small intestine must produce adequate amounts of the enzyme called lactase.
Lactose is found in:
Lactase is responsible for breaking down the lactose into glucose and galactose, so the body can absorb it. When the body’s ability to make lactase diminishes, the result is lactose intolerance.
It is important to note that not all dairy products cause these unpleasant symptoms of lactose intolerance. In fact, yogurt or kefir with live active cultures typically do not produce these symptoms, as the active cultures help to break down lactose prior to consumption. Also, the longer the food is fermented, the less the lactose content will be, as the healthy probiotics survive by eating the lactose sugar.

Lactose Intolerance Causes

What triggers lactose intolerance? As described above, lactose intolerance is caused by the body’s inability to effectively digest lactose due to malabsorption or low levels of lactase produced in the digestive tract. This seems to occur for several main reasons:

1. Genetics/Family History

While it has been documented only rarely, the inability to produce lactase can sometimes be congenital. Researchers believe there are genetic links to lactose intolerance causing symptoms to appear during the teenage years. However, just because you made it through your teen years without affliction doesn’t mean you are immune for life. Lactose intolerance is not very common in children under two years of age, although it’s still possible.
In addition, lactose intolerance seems to run in families, and certain ethnic groups have greater occurrences of lactose intolerance than others. Native Americans, Hispanics, Asians and those from African descent often experience intolerance more often than those of European descent.

2. Aging

As we age lactase production decreases, leading to intolerance in individuals who otherwise never had overt signs of lactose intolerance.

3. Illness and Stress

In some cases, lactose intolerance can also result from surgery, injury, illness and even certain treatments. Common conditions that can contribute include gastroenteritis, IBS, Crohn’s Disease, ulcerative colitis, celiac disease and other conditions of the digestive tract, including candida overgrowth and leaky gut. Even cases of the flu can cause intolerance (however, often the symptoms will fade over time). Additionally, taking medications including some antibiotics for extended periods of time may disrupt gut health and contribute to lactose intolerance.

Diagnosis

How do doctors test for lactose intolerance? To test for lactose intolerance in patients who are experiencing symptoms like bloating and diarrhea, doctors rely on a number of different tests, including:
Keep in mind that a number of other conditions aside from lactose intolerance can cause similar symptoms. These include: irritable bowel syndrome, celiac disease, inflammatory bowel disease or SIBO (small bowel bacterial overgrowth). This is why doctors must rule out these causes before confirming a diagnosis of lactose intolerance.

Symptoms of Lactose Intolerance

What are symptoms of being lactose intolerant? The most common symptoms of lactose intolerance include:
When do lactose intolerance symptoms start? These warning signs of lactose intolerance can arise anywhere from 30 minutes to two hours after the consumption of dairy products and can range from mild to severe. Most immediate reactions are caused by the body not having the enzymes to digest the lactose sugar, which causes the intestines to contract.
If you have had an ongoing intolerance, you might also experience issues besides digestive upset, such as more extreme headaches, migraines or bloating that can occur over the course of up to two days from these undigested particles entering your body, especially if you have leaky gut syndrome.
Can you become lactose intolerant all of a sudden? This is more common among older adults, but usually lactose intolerance is obvious from an earlier age.
How long do lactose intolerance symptoms last? The severity of lactose intolerance symptoms depends upon personal tolerations and the amount consumed. If you’re intolerant and continue consuming lactose without making any other changes, your symptoms will likely persist.

Lactose Intolerance Treatment & Diet

There is currently no permanent cure for lactose intolerance because no treatment can increase the amount of lactase your small intestine makes. However, there are steps to take to manage symptoms and avoid complications. One major concern for people who have lactose intolerance is they may not get enough of the essential nutrients found in milk products, including calcium, magnesium, vitamin D and vitamin K, for example.
While it’s an option to take dietary supplements called lactase products that help digest lactose, this will not solve the underlying problem and may not be a good long-term solution.
What foods should you avoid if you are lactose intolerant? Do you necessarily need to give up all dairy?
Depending on the severity of your intolerance, it may be necessary to take a break from dairy while you heal; however, by following a healthy lactose intolerance diet, it may not have to be a permanent sacrifice.
Some people with severe lactose intolerance will need to avoid having most or all dairy products. Others can tolerate certain kinds without experiencing a flare-up of lactose intolerance symptoms. For example, some research suggests that many people with lactose intolerance can have up to 12 grams of lactose, the amount in about 1 cup of milk, without triggering any strong symptoms. Some experts also believe that one key to consuming dairy products while eating a lactose intolerance diet is to choose raw and unpasteurized products made from raw cow, goat and sheep milk.
A study published in the Journal of the Dietetic Association indicates that consuming kefir improves lactose digestion and tolerance. Participants in the study perceived a reduction in the severity of gas by 54 to 71 percent. While kefir is a dairy product, the fermentation process breaks down the naturally occurring lactose, making it easier for the body to digest and absorb it. The result is that the majority of individuals with lactose intolerance can still enjoy some types of dairy, while reaping the health benefits.
If you need to avoid all lactose, keep a careful eye out for dairy derivatives that hide in common foods — including bread, pastries, crackers, cereals, soups, processed meats, protein bars and candy. Look at ingredient labels and avoid these foods as much as necessary to control your symptoms:
There is no FDA definition for the terms “lactose-free” or “lactose-reduced.” Even products advertised as “non-dairy” could contain trace amounts of dairy products that can lead to the disrupting symptoms of lactose intolerance. Additionally, healthy natural foods that you have eaten for years may be at the root of your lactose intolerance. When transitioning to a lactose intolerance diet, it is important to carefully read the labels of all processed foods to ensure dairy products aren’t lurking.
Ideally, the best dairy products to consume if you have lactose intolerance are the types made from raw cow or goat’s milk that have been fermented for a minimum of 24 hours.

Below are additional steps to take to help manage lactose intolerance:

1. Use Organic Fermented Dairy

Fermented dairy improves the digestibility of the lactose, fats and protein in dairy, but also helps to spur healthy digestion of other foods. While the idea of drinking fermented dairy may be off-putting to some, high-quality, organic kefir is slightly tangy, creamy and ultimately satisfying.
It is similar to yogurt, just thinner and drinkable. Probiotic foods are rich in vitamins, minerals and essential amino acids. Kefir contains high levels of thiamin, B12, folate and the secret bone-builder, vitamin K.
Vitamin K2 specifically helps calcium to metabolize, creating stronger bones, which is essential to people on a lactose intolerance diet. Organic fermented dairy also helps to increase magnesium levels. Magnesium deficiency is common in people with digestive tract disorders, including celiac and Crohn’s disease and IBS … as well as lactose intolerance.
You may choose to eliminate all dairy products for a time to help reduce symptoms and help your body heal, but ideally you can begin to swap out regular dairy for fermented dairy, which can help to restore the health of the digestive tract and has enzymes that will actually aid in digestion.

2. Try Goat Milk

For many people, goat milk may be easier on the digestive system than cow milk. Goat milk is high in fatty acids, and it is more easily absorbed and assimilated in the body. The actual fat particles in goat milk are smaller and contain lower concentrations of lactose.
It takes significantly shorter time to digest goat milk products than it does cow milk products. And yet, goat milk is richer in calcium, phosphorus, iodine, potassium, biotin and pantothenic acid. In addition, its casein levels are reduced, making it friendly to those with casein sensitivity.

3. Take Digestive Enzymes That Contain Lactase

Lactase is the enzyme that is lacking in the digestive tract for individuals suffering from lactose intolerance. According to a study published in the Alternative Medicine Review, digestive enzyme supplementation can aid in the breakdown of fats, carbs and proteins, assisting in efficient digestive function
Taking specially formulated digestive supplements provide a safe treatment for digestive malabsorption disorders, including lactose intolerance.
Take a digestive enzyme at the beginning of each meal, to ensure that foods are fully digested. This also helps to decrease the probability that partially digested foods including proteins, fats and carbohydrates will sit in the gut.

4. Supplement with Probiotics

This is an essential part of a lactose intolerance diet. The live or active cultures in yogurt, kefir, fermented vegetables and supplements help to maintain a healthy digestive tract. Increasing healthy bacteria in your gut may help to spur greater lactase production, or at the very least, aid in digestion.
By adding probiotic supplements and probiotic-rich foods to your diet, you can change the balance in the gut, leading to greater nutrient absorption. Managing lactose intolerance with yogurt and probiotics is possible, according to a study published in the Journal of Applied Microbiology.
However, probiotic supplements can do significantly more for overall health and wellness than just gut health. In fact, according to a study published in Science Daily lead by Dr. Collin Hill from the University of College Cork in Ireland, probiotics may be used in the future to help control disease, without relying on antibiotics.
It is important to look for a supplement that contain probiotics plus prebiotics derived from heat resistant soil-based organisms.

5. Incorporate Calcium-Rich Foods

While calcium is often considered a powerful mineral in the fight against osteoporosis, it is much more vital to our health than just our bones. In fact, calcium-rich foods help promote heart health and manage body weight. Calcium rich foods, which everyone should incorporate in their lactose intolerance diet include raw milk, yogurt, kefir, dark greens like cooked kale, raw cheese, sardines and broccoli.

6. Add Foods Rich in Vitamin K

As mentioned above, vitamin K plays a major role in calcium absorption and bone health, but its benefits do not end there. It also helps promote brain functioning and improve insulin sensitivity. This fat-soluble vitamin is stored in the liver, and proper levels can be disrupted by antibiotic use, certain prescription cholesterol medications and IBS and leaky gut. Many people who are lactose intolerant are also vitamin K deficient, so it is important to make sure you are getting enough in your daily food routine.
Foods rich in vitamin K to add to your lactose intolerance diet include green leafy vegetables, scallions, Brussels sprouts, cabbage, broccoli, cucumbers and dried basil. In addition, fermented, organic dairy is also rich with this essential vitamin.

7. Add Bone Broth to Your Diet

Central to helping restore the gut is bone broth. This simple and tasty broth helps the body overcome food intolerances, sensitivities and even allergies, while improving joint health, boosting the immune system and reducing cellulite.
Long simmering of grass-fed beef bones or organic free-range chicken transforms the calcium, magnesium, phosphorus, sulfur and other minerals, making them easier to absorb. In addition, the natural collagen and gelatin found in the bones help to support the GI tract. Aim to consume 8 ounces to 12 ounces each day.

8. Jumpstart Your Gut Health with the GAPS Diet

The GAPS diet plan was designed by Dr. Campbell to help reduce inflammation, treat autoimmune diseases, support healthy neurological function and minimize digestive disorders. If you have experienced the symptoms of lactose intolerance for months, or years, you can jumpstart your transition by following this eating plan.
The foods consumed include many of those mentioned above, like raw fermented dairy, fruits and vegetables rich in vitamins and minerals, healthy nuts and beans, wild fish, grass-fed beef and free-range chicken.

9. Add Non-Dairy, Probiotic-Rich Foods to Your Diet

Probiotic-rich foods increase the overall health of the digestive system and can help ease common digestive upset symptoms (including poor nutrient absorption), strengthen the immune system, support weight loss and increase energy due to more vitamin B12 in the body.
Sauerkraut and kimchi are both made from fermented cabbage and other vegetables that are nutrient rich, and rich with enzymes that help digest foods. Probiotic drinks, including kvass and kombucha, are rich with healthy bacteria, which help with liver detoxification, along with coconut kefir.
Coconut kefir is easy to make at home with the same types of kefir grains used in dairy kefirs and is rich with the healthy bacteria found in organic fermented dairy products.

10. Use Coconut Oil for Cooking

Coconut oil is one of the most amazing foods on the planet, and is easily converted to energy in the body. In addition, it helps to improve digestion, burn fat, kill bad bacteria and fungus and regulate candida in the body. Coconut oil can be used for high-heat cooking, it can replace dairy in coffee and tea and it is easy to bake with. It helps to fight inflammation throughout the body, boost the immune system and even prevent bone loss. For individuals that are limiting their traditional dairy intake, coconut oil should be included in their diet.

11. Substitute Ghee for Butter

Ghee has been used for thousands of years to improve digestion function, reduce inflammation, support weight loss, strengthen bones and so much more. But the most important factor for individuals with lactose intolerance — ghee contains only trace amounts of lactose that most aren’t likely to react to. The long simmering process and skimming of the butter removes most lactose and casein, so individuals with sensitivity or allergies to dairy products should try ghee. In addition, when created from milk from grass-fed cows, levels of conjugated linoleic acid or CLA, are double or triple that of traditional grain-fed cows.
Ghee is versatile and can be used for everything from high-heat cooking to “buttering” toast. Like coconut oil, ghee is part of my healing foods diet.

Final Thoughts

The Best Milk Alternatives, According to a Dietitian
Plant-based alternatives to cow’s milk have been a thing for a while now, and the benefit of this trend is that there’s something for everyone. Whether you’re lactose-free, vegan, or allergic to tree nuts, soy, or coconut, grocery stores in 2019 definitely have something to suit your needs. But how are you supposed to navigate the growing non-dairy aisle? And are these milk alternatives any healthier than cow’s milk?
When it comes to nutrients, commercially available versions of nut, seed, and legume-based milks contain varying amounts of protein and fiber (usually 1-4g each per 1-cup serving) since they’re made by blending the predominant ingredient with water. They can contain around 100 calories or less depending on the ratio.
Consuming fewer calories can be a good thing if you’re guzzling iced coffee with unsweetened almond milk all day, but may be less desirable if you’re looking for a nutritious addition to your morning bowl of steel-cut oats. Many of these alternative milks also cost more than dairy milk, but they may be worth the spend depending on how often and where you’re using them.
As a registered dietitian, my main piece of advice is this: Don’t be swayed by trendy marketing claims on packaging. Depending on the brand and type, these milk substitutes can be sneaky sources of added sugar, so it’s crucial to check labels before you swap out a dairy-based option for a plant-based one. Here’s everything else you should be scanning the label for.

What to Look for in Alternative Milks

At least 7-8g protein per serving
As few ingredients as possible
The word “unsweetened” and “0g added sugar”
Limited saturated fat (especially in ones made with coconut or added protein)
Less than 140mg of sodium per cup
Fortification with calcium and vitamin D
Nutrients you’re personally concerned about (like the omega-3’s)
The best milk alternatives are typically unsweetened soy or pea-based blends (yep, you read that right!) that are fortified with calcium and vitamin D. These two nutrients are better absorbed when consumed together and are beneficial for strong bones, hormone regulation, and general immunity — especially for your little ones. If you’re adhering to a vegan diet, you’ll want to look for blends that have vitamin B12, vitamin A, and DHA/EPA omega-3’s, as well. Be sure to skip “barista blend” milk alternatives. They’re better for frothing but often come with loads of added sugar. Instead, spice up a hot beverage with vanilla, clove, or a cinnamon stick.
Keep reading to get the low-down on the best plant-based milks you can buy (and order):

1. Soy

Arguably the OG milk substitute (and the most nutritionally similar to dairy milk), unsweetened soy packs about 80 calories per cup with 8g plant-based protein from soybeans. Soy milk is made by soaking and blending these little beans and straining out the leftover pulp before consuming. Filled with antioxidants and fiber, soy alternatives are often super nutritious and provide key polyunsaturated fats.

2. Pea Milk

A new kid on the block in the world of plant-based milks, pea milk is made from pea protein isolate, water, and other emulsifiers like algal oil, sunflower oil, and guar and gellan gums. It’s as creamy as soy with a slightly less nutty taste for 70 calories per cup. The use of algal oil provides DHA, a key omega-3 fatty acid that’s linked to immunity, heart health, and cognition. The unsweetened versions pack up to 8g protein from a nutrient-dense source.

3. Coconut Milk

Coconut milk is made from water and coconut cream (VitaCoco’s uses coconut water, too), so it has a tropical taste compared to other plant-based milks. Nutritionally, coconut milk is higher in fat and lower in carbohydrates than nut- or grain-based milks. Most of the calories come from saturated fat — just one cup has up to 4g, which is 20% of your daily value. That said, the creamy consistency and fat content help boost satiety, so you’re likely to use less of it — especially if you’re adding it to coffee and tea.

4. Oat Milk

The trendiest blend of the bunch, oat milk is a creamy, lightly flavored addition to coffee, tea, cereal or a homemade smoothie. It contains added fiber, which may make it more filling than other alternative milks, according to early research. That said, oat milk is lower in protein than non-fat cow’s milk or soy-based versions (2-4g versus 8g per cup). It also has slightly more calories than unsweetened almond milk, which can add up if you swig it frequently.

5 Almond Milk

Most commercial almond milks range between 35-90 calories per cup and there are loads of blends and unsweetened versions to choose from. They’re mostly made from almonds and water, plus other emulsifiers and fortifying nutrients. The lower-cal versions give you about 1g each of protein and fiber per serving (though Elmhurst’s blend is about 5g protein). The low protein content is something to keep in mind if you’re using almond milk as a dairy swap in homemade smoothies — you may want to pump up the protein by adding nut butter or chopped nuts.

6. Cashew Milk

Cashew milk is particularly tasty in tea or homemade tea lattes. Try it with matcha for a little midday boost of L-theanine, a compound found in matcha which is linked to cognition and focus. Cashew milk is made the same way as almond (soaking, blending with water, and straining) and is similar in nutritional composition, ranging from around 40-50 calories per cup. Cashews themselves provide zinc, copper and magnesium, which help support your immune system. The real difference between cashew and almond milk? The flavor! Go with whichever you prefer, so long as you’re choosing an unsweetened version.

7. Peanut Milk

Peanuts are like the crown jewel of foods, since they’re good for both you and the planet. They have a similar taste and nutrient profile as tree nuts (almonds, walnuts, cashews, and hazelnuts), but these legumes actually grow underground and use way less water. The best thing about peanut-based products is they’re often more cost-effective than other dairy alternatives and are higher in protein (plus, they have a creamy taste and texture).

8. Flax Milk

At 70 calories per cup, flax milk contains a little more than meets the eye. Most store-bought versions are made from a combination of water, flaxseed oil, and pea protein, which makes it similar in nutrient composition to pea milk. The alpha-linolenic acid found in flax also helps support immunity and has been linked to reducing the risk of heart disease.

9. Hemp Milk

Out of all of the hemp products currently on the market, hemp milk is a solid choice from a nutritional POV. It’s made by blending hulled hemp seeds with water, and packs magnesium, calcium, and vitamin D, depending on fortification. You’ll also get omega-3 and omega-6 fatty acids — essential nutrients for your immune system and cognition — plus about 3g of protein at 60 calories per cup. However, there’s very little fiber in hemp milk compared to ones made from other seeds. The main objection to hemp milk is the taste: Its ultra-nutty flavor can be bitter to some, especially if you’re used to the sweeter notes of other plant-based alternatives.

10. Rice Milk

Rice milk is made by blending rice with water. It’s often lower in calories than other milk alternatives in its unsweetened form, but since the flavor is very mild, most versions contain added sugar. You may be better off with an alternative grain blend unless you’re avoiding nuts, seeds, or legumes because of an allergy.

11. Walnut Milk

Walnut milk is a top pick if you’re looking to boost your intake of plant-based omega-3’s. It tastes a little more earthy than other types of milks and packs 3g of plant-based protein for 120 calories. Use it in tea or coffee to shake up your morning routine, or in smoothies to balance out sweet-tasting fruits.
Source: http://www.hiwamag.com/health/7-symptoms-lactose-intolerance-plus-11-tips-manage/
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[OC] Corridors - Chapter 23: Schism (Part 2)

This is Part 2! You can find Part 1 here
“Alright, Wrixea, but I hope you have a good plan!” As if in response, clouds of Kredith Worker ships started streaming out of the remaining Hiveseeds in solar orbit, rotating their small chassis to orient their double-helical points in the direction of the incoming Forsaken vessels. Pools of light gathered behind them as they powered up their interstellar engines and hurled themselves past the Forsaken fleet. “Where are they going?” Alan asked incredulously. He scattered a dozen probes into the Ekres star below, unleashing a furious maelstrom of ravenous flames that consumed almost a fifth of the Forsaken ships in orbit over Ekres IV.
“What the fuck are those?” he asked aloud as his tactical overlay fluttered rapidly with thousands and thousands of small detonations, occuring just behind the Forsaken fleet around the star. Alan frowned as icons denoting navigational hazards filled the screen, carpeting the space behind the Forsaken forces. Another swarm of Kredith worker ships raced past his Blinkship, trailing streams of light in their wakes as followed their brethren into superspace. Detonations appeared on his overlay again, quickly replaced by increasing numbers of hazard icons. “Wrixea, what are those worker ships doing?”
Several purple explosions suddenly bloomed in the distance as Forsaken Dreadnoughts dropped into normal space in several large pieces which immediately ignited in quick succession. Voidblades spun into existence with severed wings and large gashes carved in their hulls, instantly exploding upon rematerialization. Alan widened his eyes in shock as he realized what was happening, “Wrixea, your worker ships are self-destructing in superspace!?”
“Correct, Pilot Radisson. Their overloaded interstellar engines will create temporary ruptures in superspace, preventing the Forsaken from utilizing their intended trajectories. The enemy will be destroyed as they fly through the ruptures, and we will easily reassert our claim over the Ekres Star as they mindlessly destroy themselves.” Colonykeeper Wrixea gnashed her mandibles triumphantly, waving her antennae in excitement, “They will need to re-calculate their approach vectors to compensate, but we will constantly saturate their new courses with more superspace ruptures!”
Alan watched as another group of Dreadnoughts dropped into normal space, spinning chaotically into each other and exploding. Purple fire threw itself across the tainted space, continuously flaring up as already-destroyed Forsaken vessels arrived and summarily shattered. “Are you sure this is the right thing to do, Wrixea? Are the Mindweavers sure about this?”
“To control the enemy’s movements is to control their fate! The Mindweavers learned this from you, Pilot Radisson.” Colonykeeper Wrixea proclaimed with pride. She flaired her wings emphatically, “And because of your tutelage, we shall be victorious today!”
Alan couldn’t argue with the efficacy of their new battleplan, but the way the Kredith cavalierly spent the lives of their workers gnawed at him. “But your workers… You’ve already sent over fifty thousand Kredith to their deaths, just to close off one approach vector.”
“Do not worry about the Worker drones. Their purpose is to serve the Kredith Dominion and ensure the survival of the Hiveseeds. And in this way, they have succeeded.” Colonykeeper Wrixea dismissed with a wave of her upper left insectoid limb, “New worker bodies will be rebuilt, and we will infuse them with the minds lost in these maneuvers. Nothing of value is lost, save for a small amount of biomass.”
The Hiveseeds in solar orbit fired their rivers of ion bursts with renewed vigor, pummeling what remained of the Forsaken fleet into charred and burning twisted metal. Shadowspikes ignited, vainly announcing their demise with weak flares as the Hiveseeds buried them with endless torrential ion fire. Without being harried by the Shadowspikes, the Blinkships closed in on Ekres again, littering the star’s surface with Pathfinder Probes to transport its rage to their chosen targets. Sunbursts ignited over the skies of both Ekres IV and V, annihilating the Forsaken vessels in orbit with dancing flames. Onathin Nestships poured an onslaught of photon lances into the dark fleets, slicing white-hot incisions into their black hulls and spilling their insides into the vacuum of space. Purple flares peppered the battlespace, dwarfed by sudden brilliant orbs of light as Pathfinder Probes endlessly ejected coronal mass into the Forsaken hordes.
Flockleader Wiksen chirped into the open communications channel, “Half of my forces have been destroyed, but it appears that the Forsaken numbers are thinning out. Perhaps they have reconsidered their invasion plans, in light of your recent adaptations.”
“Good, because we’re running out of Pathfinder Probes.” Alan warned through gritted teeth as he narrowly dodged a whiplash of curling flame from the star’s surface, “If they don’t let up soon –” His tactical overlay suddenly blared at him as the red signatures seemed to rotate in place, before streaming out of the Ekres Star System. “They’re retreating!”
“A victory well-earned and fought!” Colonykeeper Wrixea flapped her wings and screeched.
“I thought they had a lot more ships they could throw at us?” Alan asked, “But I’m glad they decided to retreat. It gives us time to get resupplied, and maybe our capital ship can be here for the next battle!”
Flockleader Wiksen’s feathered face suddenly appeared on the viewscreen. Singed feathers stuck out awkwardly all over his wings, and lines of green throbbed throughout his feathers from adrenaline. Both pairs of eyes drooped in stress and fatigue, and held a heavy despair within them, “You are correct, Alan Radisson. The Forsaken do have more ships, and they are sending them from their Voidbase directly to the Orkina System.”
“What?!” Alan shouted as he pulled up the long range telemetry.
“This invasion was a farce, meant to pin our forces in Ekres while they begin the assault unhindered in Orkina. We suspected they had the range necessary to reach Orkina, but with insufficient data, and the fact that the defense of Ekres was paramount…” Wiksen covered his face with a wing and shuddered, “I’ve doomed my people to defend others!”
“We shall mobilize all of our forces immediately to defend Orkina System!” Colonykeeper Wrixea exclaimed.
“Doing so would leave your Mindweavers vulnerable!” Flockleader Wiksen jabbed his talons at a screen, “Even after the success of this battle, the Forsaken have enough ships to triple the entire allied fleet! The ships left behind at their Voidbase doubles the fleet sent to attack Orkina! If we leave Orkina, they will come here and destroy the Mindweavers. There is nothing we can do to help Orkina.”
“Then we shall leave Ekres! We will not be able to reach Orkina in time to mount a successful defense, but perhaps if we rally at the Henfir System, like General Davis has suggested, we can resist further Forsaken incursion into the Onathin Sovereignty!” Colonykeeper Wrixea proposed.
“Abandon Ekres? But your Mindweavers may die from being uprooted yet again.” Flockleader Wiksen flapped his wings, “And we cannot accommodate them in Henfir.”
“They will adapt!” Wrixea insisted, “We cannot allow Onathins to suffer at the hands of the Forsaken like the Kredith already have. The Mindweavers will not tolerate injustices inflicted upon a people that have defended us to such a large extent! Abandoning Ekres is trivial. The Kredith Dominion is not made of worlds. As long as we have the Mindweavers, everything can be rebuilt and replaced! In any case, the defense of Ekres is untenable in the longer term if we are severed from the Onathin Sovereignty!”
“Well said, Colonykeeper,” Alan agreed. “I’m ordering my Blinkships to launch probes to Henfir now. I just hope we get there in time.”
The brightly lit, pristine corridors and hallways of the North American Branch of Earth Council contrasted sharply with Ambassador Evans’s mood as he made his way past them. His usual hopeful outlook on life was marred by the current state of affairs. Although Ekres had repelled the Forsaken incursion, the situation in the Onathin Sovereignty was still spiralling wildly out of control. If he were to be of any help, he’d have to start forging stronger links with the outlying Onathin systems. Months and months of Pathfinder Probe-accelerated trade amongst these systems, and with Earth, gave the Onathins in those systems an immense appreciation for humans. Perhaps there is a way I could secure these economic ties further, and form some sort of mutual defense pact? That might take some of the pressure off of Prelate Iwardion’s back. Rubbing his eyes in fatigue, Tyler retrieved his tablet from his pocket and ordered a taxi-drone to deliver him to the Vancouver Space Elevator.
His eyebrow twitched in confusion as threads of colour wafted idly throughout the hallway. Tyler curiously approached the source of the Drikenyl song and realized it was coming from Tara Yang’s infirmary. He stepped through the threshold and waved at the pair of Drikenyl who lazily floated in the water-filled observation port located on the wall directly opposite of the entrance. They twirled their whiskers in response and continued to sing, casting shimmering waves of blue and green into the room.
“Hello Tyler,” Tara Yang greeted as she noticed him wander into her infirmary, “Are you injured?” She walked over and automatically began examining him with her practiced eyes.
“No, I’m fine.” He recognized both of the Drikenyl floating beyond the glass wall. One of them had much more luxuriously reflective scales, and was the Drikenyl that they had picked up from Sechalla Station. It was the first Drikenyl ever to arrive on Earth, a fact that it liked to show off by flashing its nourished scales ostentatiously. The other Drikenyl had been singing to him when he had woken up in Tara’s infirmary a couple of weeks ago. “What about them? Are they infected with that bacteria that’s been spreading amongst the Pilgrim Drikenyl?”
“No, they’re OK. They’ve got a very effective quarantine system set up above the Salish Sea, isolating infected Drikenyl into those levitating spheres of water.” Tara took out her tablet, reading a notification that had just popped up, “I’m not sure why they’re still here, but I’m actually very close to perfecting an antibiotic that’ll be effective against it.”
“That’s good to hear, Tara.” Ambassador Evans said as his eyes wandered throughout the room. Hues of blue and green soaked through the glass window-wall and wrapped themselves around the infirmary beds, surgical armatures, Onathin laboratory equipment, before entwining around the people within the room. At the far end of the infirmary, Derek was inspecting a piece of Drikenyl hide. Rainbows flashed across his face as he fiddled with the iridescent scales. He reached up with a free hand and rapidly scratched the side of his head.
“Derek, don’t scratch like that! You’re going to inflame your scalp.” Tara chided from her lab bench, “Anyway, the bacteria infecting the Drikenyl is partly of Earth origin. That bacterial conjugation image that you keep getting from the Drikenyl is probably what happened between two bacteria, one of Drikenyl origin, and one of Earth origin, that were both benign. But together, they produced a pathogenic progeny.”
“Wait, so two usually good bacteria mated and produced bad bacteria?” Tyler clarified with raised eyebrows, “Does that happen a lot? And what are the chances that Drikenyl bacteria could mate with Earth bacteria?”
“I guess I was a little imprecise. The ‘Drikenyl bacteria’ was also originally from Earth, but changed and evolved inside the Drikenyl gastrointestinal tract due to their unique biochemical environment. Then, it randomly conjugated with another Earthborne bacteria found in the ocean. It’s a random process, and almost impossible to predict.” Tara placed the tablet down on a nearby desk and stretched while yawning, “I’m actually quite surprised at my progress! I expected this to take a lot longer, but ideas just kept popping into my head.” Tyler eyed the wisps of colour that misted around her head and smirked, “I guess your muse was working overtime.” He waved at Cerion in a corner of the lab, who waved a wing in response.
“Ambassador Evans, it is good to see you again. Have you heard any news regarding the Tymin System and my parents?” Cerion asked as she scratched at the polymer interface of the Onathin machine in front of her. A small whir seeped into the room as a spherical, glass component of the machine began to spin, casting ripples through the blue-green aura that filled the room.
“I’m sorry, Cerion. All communication with that part of the Sovereignty has been severed since Vyndres and Trennor seceded.” Ambassador Evans replied sadly, “We’re working on getting corridors established with those outlying systems, so we’ll have more news soon.”
Cerion’s crest feathers deflated, but she nodded and blinked her thanks, “Please inform me when you have any news. My parents are all I have left.”
“Absolutely.” Tyler waved at the Onathin lab equipment, eager to change the subject, “and how has the cure for the neural parasite been proceeding?”
“We’ve actually put that on hold for now.” Tara answered, “We’re still working on the parasite, but we’ve decided to focus our efforts on disrupting the neural network that the parasite forms in the Stalwart Claw hosts’ brains. If we can disrupt the network, or suppress the parasite’s ability to form these networks, I believe we can destroy its mind control abilities.”
“So you’re starting over from scratch?” Tyler asked.
“Actually, we are much, much closer to a network disruptant than an outright cure!” Tara said with excitement flashing in her eyes, “On Gorandis, when were performing high-throughput drug screening on the neural parasite, we couldn’t find any drugs that could kill the parasite in a dose that’s tolerable to Onathins. However, we found many drugs that caused the parasite to retract its pseudopodia!” “False feet?” Tyler quirked an eyebrow.
“Exactly!” Tara continued, “The parasites extend appendage structures from the rest of their body called pseudopodia, which they then use to bind with other parasites and form plaques in the brain. They also use these appendages to anchor themselves into the Onathin brain and influence neural patterns. I think that the Stalwart Claw strain is able to extend their pseudopodia to much longer lengths than the other strains, which allows them to build this neural network and gain complete neural control of the host!”
“Because of our efforts on Gorandis, we have plenty of candidates to test for their network disruption capabilities,” Cerion echoed, “And while a cure is still preferable, this may be the fastest and most time-efficient way to restore stability to my civilization. If the Stalwart Claws were to suddenly awaken from parasitic control, they would see the error of their ways and strive to unite the Sovereignty once more!”
“I hope you’re right.” Ambassador Evans rubbed his eyes again, “The situation is getting a lot worse. The Finsen Star System just voted to secede from the Sovereignty, and there’s been numerous protests and demonstrations on Onathi itself. Prelate Iwardion has invoked the Sovereignty Security Provision, bypassing some universal rights and instituting a Sovereignty-wide detainment of any Stalwart Claw affiliated Onathin,” He sighed, “Several analysts are predicting that a coup attempt on the Onathin Homeworld is likely in the next few weeks.”
Tara pursed her lips in worry, “I’m not sure if we will have something before then.”
“Is there something I can get you from the Onathin Sovereignty? Something that I could ask Steward Gredion to ship over from Sechalla Station that might speed up the project?”
Tara and Cerion exchanged glances, “Well, we need test subjects.”
“Test subjects?”
“At least samples of the parasite spore, and they have to be that specific strain of parasite that’s infecting the Stalwart Claws. We need the strain that’s forming the neural network and controlling their minds, so we can test our drugs for their neural network disruption efficacy.”
Ambassador Evans shook his head, “I’m not sure I can provide that. Maybe Steward Gredion arrested a saboteur aboard Sechalla Station that might carry the right type of parasite that you need. Diplomat Pellon might be better suited to acquiring Stalwart Claw prisoners, but…” he looked down at his tablet in worry, “I haven’t been able to contact him for the past couple of weeks. He…was in the Brildin Nexus Relay when the Stalwart Claws attacked.”
“I’m sure he’s fine, Tyler.” Tara said quickly, “He is a very resourceful fellow. He must have found a way to survive.”
“We’ll see.” Tyler glanced at the tablet and noticed that the taxi-drone he ordered was approaching the building, “If I see him, I’ll let him know what you need.”
“Where are you going?” Tara asked, eyeing Tyler’s tablet, “Not into Sovereignty Space?! It’s pretty dangerous for any human, ambassador or not!”
“I’m heading off to all of the outlying systems that we’ve been trading with. Don’t worry, none of them are in any danger of secession, and they all have very positive attitudes towards humans. I’ll be fine.”
“Still, be careful.” Worry lines etched across her face as Tara looked at Tyler, then at Derek, “I don’t want to lose another person I care about.”
Derek, oblivious to Tara’s worried stare, brandished his omni-tool and jabbed at the Drikenyl hide. With a soft plink sound, the nanite tool glanced off the scales and embedded itself into the table underneath. He frowned, and stood up straight as if to get a better view of the situation from above. A ping sounded from Tara’s tablet, sending ripples through the blue-green strands that stretched throughout the room. Tyler stared at the tinted filaments around Derek's head, wondering just what exactly the Drikenyl were doing, when Tara suddenly gasped.
“Cerion! Tyler!” She said excitedly, “The aerosol sample contains the strain of the parasite that we need!”
“What aerosol sample?” Tyler asked confusedly.
“Prelate Iwardion sent over a sample of an aerosol that the Stalwart Claws were disseminating on several core worlds, as well as a dozen outlying systems. It arrived a few days ago, and my colleagues at the European Branch of Earth Council have identified it as a compound that contains the specific parasite strain that infects the Stalwart Claws.” Tara scrolled through the data on her tablet, “It also contains several compounds that I’m not familiar with. The Europeans suspect that they’re bonding agents, but they’re not sure. Cerion, what do you think?”
The blue-feathered Onathin waddled over to Tara and peered her four eyes at the tablet. Two of them narrowed, “These compounds are derivatives of molecules commonly used to allow drugs to penetrate the primary airflow sieves that line the initial airway tract in Onathins. We use these compounds when we want patients to breathe in drugs that would immediately accumulate in the cranial vasculature.”
“What does that mean?”
“It means that the Stalwart Claws are trying to get other Onathins to breathe their specific parasite strain directly into their brains.” Tara answered grimly. “They’re poisoning their own people. Or rather, they’re trying to make more of themselves.”
“But I thought all Onathins were already infected with the neural parasite? How does this work?” Tyler asked as he stared at Tara’s tablet. Graphs and charts scrolled endlessly across the screen, unintelligible to him.
Tara nodded, “You’re right: all Onathins are already infected. But even so, the Stalwart Claw strain is undoubtedly more belligerent, and would eventually outcompete the parasites that are already present in the host.” She tapped a nearby monitor, showing the schematic of the neural mesh that the parasites form, “The neural network that the Stalwart Claw parasites build allow them to be hardier, enhance their rate of nutrient uptake, and to reproduce faster. Imagine a town. It’s easier for families to survive in their individual houses when there is a network of roads and streets to allow them to gather resources and remove wastes.” She jabbed at her tablet with a furious finger, “They’re trying to accelerate the spread of their strain with these aerosols. It’s all a part of their plan to take over the Sovereignty.”
“But herein lies our solution, Tara Yang!” Cerion chittered, “We now have a sample of Stalwart Claw parasites that they have supplemented to grow networks more rapidly. We can use these to test our formulations, and successfully devise a neural disruptant!”
Tara beamed at the both of them, “My thoughts exactly! Cerion, please spool up the zwitterionic stabilizer. We can start manufacturing Onathin drugs while we wait for the parasite sample to ship in from Europe.”
Tyler caught a faint rustle from the corner of his eye. A tear in the fabric of colour around them bounced around the room, distorting the otherwise smooth blue-green air. “What’s wrong?” he asked aloud as the Drikenyl started to flick their scales back and forth in agitation.
The enemy have advanced their forces. the First Drikenyl resonated.
Tyler’s tablet vibrated as it repeatedly sent pings crashing into the cerulean waves that were slowly receding from the room. He took it out under both Cerion and Tara’s stare, and deliberately flicked it on. A newscaster was shakily reading out the report in front of her, “Forsaken forces…have bypassed the Ekres blockade and have entered the neighbouring Orkina System. Onathin ships are scrambling to fend off the Forsaken, but there’s…there’s just so many of them.”
The newscaster popped up on one of the screens that stretched across one of the laboratory walls, “Civilian ships that are already in space are quickly evacuating, but there are still hundreds of millions of Onathins on the surface of the planet!” Tyler winced as light flickered harshly from the screen. The feed from an orbital satellite above Orkina II replaced the newscaster’s stricken face, displaying hundreds of small, sleek Onathin ships streaking away from the planet. Rivers of light bloomed from behind their slender silver bodies as they spooled up their interstellar engines and raced out of the system. Dark crescents loomed into view from the edge of the feed, reaching out with maroon lasers, hoping to slice through the evacuating transports. One of the purple beams carved across the left side of the screen, and suddenly the feed went blank. The newscaster quickly rematerialized, looking slightly more collected than before, “Reports are still coming in, but it seems that the Forsaken have amassed a fleet of at least a thousand ships to assault the Orkina System. Half of the spaceports along the western continent are still under Stalwart Claw control, and they have not lifted their launch restrictions, and are greatly limiting the rate of evacuation! We’ll have more for you after a few moments.” The newsfeed ended abruptly.
Bright green lines traced along Cerion’s feathered face as she huffed and puffed rapidly. “It…it can’t be.” She squawked involuntarily as she stumbled into a nearby chair, “I knew…I knew the Forsaken were close, and that their invasion into Sovereignty Space was a very likely possibility. I knew that it would happen. But seeing the incursion happen…I am unsure if it was ever possible to sufficiently prepare for this eventuality.” Her wings flapped subconsciously, scattering feathers across the lab equipment, and even propelling down onto the beds across the room. “What will become of my people?” she asked in quiet desolation.
Tara walked over and knelt before Cerion, studying her pulsating blood vessels. She reached up and placed a hand on her wing, “Cerion, I’m sorry that the Forsaken have invaded the Sovereignty. But we still have a lot of work to do. Now, more than ever, we need to destroy the neural parasite and re-unify your people. We still have time, but not much.” Cerion nodded feebly as Tara continued, “We must not lose hope, and we can’t give into despair. Together, we can still save lives.”
Tyler looked down at his tablet and noticed that it was still streaming a feed from the battle over Orkina II. Apparently, another orbital satellite had taken over, showing an Onathin fleet firing photons frantically at a Forsaken armada that was twice its size. The Nestships and Predator cruisers had been scattered around the planet, providing orbital support for the Sovereignty security forces battling the rebel Stalwart Claws on the surface. He raised an eyebrow, realizing that the feed was streaming specifically to his tablet, and was not being broadcasted across a newsnet. Flashing text near the bottom confirmed his suspicion.
AMBASSADORIAL ACCESS KEYS AUTHENTICATED! STREAMING… 
He watched as the orbital defense satellites spat out a phalanx of light, incinerating dozens of Voidblades and Dreadnoughts. The scattered Onathin ships had finally grouped themselves into a formation between the Forsaken fleet and the planet, defiantly throwing spears of light into the oncoming horde. Dreadnoughts exploded, replaced by even more Dreadnoughts as the Forsaken armada pressed in. Dark red plasma, underscored by purple beams of light, pummeled the Onathin ships and the orbital defense network, showering the planet below with spiralling silver wreckage.
Tyler clenched his fist and shoved the tablet back into his pocket. I guess now would be the perfect time to fulfill General Davis’s request. He strode over to the Drikenyl observation port, dimly aware of Tara’s continued condolences and attempts to coerce Cerion back to work. The pair of Drikenyl had long ceased their song and stared mournfully back at Ambassador Evans as he approached. “I want to talk to the Hierarch in my office.”
The First Drikenyl twirled its whiskers and flashed blue, As you wish, Ambassador. It flaired its wingfins, flashing brilliance once more into the room, and whirled away.
Nodding at the other Drikenyl, Ambassador Evans turned on his heel and stepped purposefully towards the exit. He passed Tara and tapped her on the shoulder, “Let me know when you have something.”
She nodded as well, and turned back to Cerion. The blue Onathin was visibly shaking, but seemed to be taking measures to calm herself. Once again, the bright hallways that playfully threw his footsteps back and forth contrasted with his dreary mood. Tyler took a deep breath as he approached the elevators and strode through the threshold. His tablet cried for his attention, insisting that he see the battle of Orkina II unfold. He ignored it, instead weaving words and sentences together in his mind, preparing for his meeting with the Hierarch. It would be the first time that he would meet the leader of the Drikenyl Warship fleet, and he was unsure of how cooperative the Hierarch would be.
His office doors whispered as he approached them, revealing a decently-sized chamber with monitors that stretched across two opposing walls. The far wall, directly opposite to the office doors, had been rebuilt with wide, thick glass panes reinforced with a thin metal net, and then connected to the Drikenyl access port that snaked upwards throughout the building. Water sat almost invisibly beyond the glass wall, surrounding a trio of Drikenyl. Two of them were hurriedly applying some sort of paste to the Drikenyl between them, tending to the many gashes, burns, skin tears, and broken scales that were strewn up and down its hide. The center Drikenyl focused all three of its eyes at Ambassador Evans, seemingly analyzing him as he walked towards the glass wall.
Ambassador Evans. I have heard of your many great deeds, and of your impressive capacity for kindness. It is an honour to finally meet the Saviour of my people. Several beveled metal rectangles, traced with pulsing blue lines, were affixed to the Drikenyl Hierarch around his eyes. Tyler surmised that they fed some sort of heads-up display to the Hierarch in times of battle, since he hadn’t ever seen any Drikenyl wear any sort of ornamentation for purely asthetic purposes. Small clear tubes, filled with orange liquid interdigitated with the Hierarch’s scales throughout its body, occasionally swelling with liquid or collapsing as it emptied its contents. The comparatively fragile wingfins were covered with an iridescent membrane, flexing with the wingfins as the Hierarch maneuvered closer to the wall. It waved its whiskers at the Drikenyl Healers, Your efforts are appreciated, and will suffice for now. Please leave us.
Very well, Hierarch. One of the Healers resonated as they fluttered back, stretched their wingfins, and whirled away.
Tyler watched the Healers disappear somewhere below the window-wall before meeting the Hierarch’s three eyes with his own, “I am humbled by the presence of a talented strategic thinker and leader such as yourself, Hierarch. No doubt, the journey to Earth was perilous with the Forsaken stalking the Pilgrim Fleet in the void.”
And it would have all been for naught, if you had not so prepared a sanctuary for my people. For that, you have my eternal gratitude. The Drikenyl flashed forest green while emitting a wave of hope that bubbled within Tyler’s chest, My apologies for our late meeting. Coordinating the disembarkation of the Pilgrims, re-energizing the fleet, and acclimating to the waters of Earth have all prevented me from seeking an audience sooner.
“I understand completely, Hierarch.” Ambassador Evans placed a hand on the glass, “And I do not want to take up too much of your time, but the situation grows desperate. I believe you are aware that the Forsaken have begun their incursion into the Onathin Sovereignty?”
Correct. It is sickening that the enemy has been able to continue their rampage towards the center of the galaxy, especially after the sacrifice of our Republic. The Hierarch rotated its top eye and inspected Ambassador Evans’s hand. After a brief pause, it mirrored the gesture by placing a forelimb against his hand through the glass. If the Enemy utilizes the same movements and strategies as they did in our war, you can expect many attacks to occur behind fortified frontier systems. They will draw your forces to the contested battleground systems, and send another fleet to attack the system behind it.
Ambassador Evans nodded, “That’s what they’re doing now. They have a Voidbase behind the Ekres stronghold system, and are currently attacking the lightly defended Orkina System.” He looked down at his tablet and sighed heavily, “Millions of Onathins are dying right now, because we were forced to fend off an attack on Ekres just hours before their attack on Orkina. There was no way we could have been able to defend both systems in such a short time span.”
This will continue to be their strategy for much of the war. They use their superior numbers and uncanny mobility to their great advantage. The Hierarch informed, The war between the Republic and the Forsaken stagnated when we adapted to their tactics. In battle, our fleets could sweep away tens of thousands of Forsaken vessels with ease. We developed technology to combat the massive numerical advantage that the Enemy enjoys, and deployed sizeable defensive fleets across many of our worlds instead of focusing solely on the frontier systems.
“The difference here is that we have neither the numbers nor the technology to defend ourselves effectively against the Forsaken. We have had success with our Pathfinder Probe weaponry, but there’s just not enough of it to hold back such a large invasion force.” Ambassador Evans thumbed on his tablet, dismissing the feed from the Orkina System battle and expanding a local starmap instead. He zoomed in the starmap on the Onathi-Henfir-Brildin-Orkina-Ekres starlane. An angry red boil seethed in the void between the Ekres and Orkina systems, indicating the Voidbase and its massive escort fleet, “The Forsaken have amassed thousands of ships here, enough to dwarf the entire allied fleet three times over. Our military analysts project that after they destroy Orkina, they are going to test the Ekres defenses again, while simultaneously attacking the Brildin System. They have enough ships to even launch an additional strike on the Finsen Star System, which is on a separate Onathin star lane. Bringing a large force to defend Finsen in such a short time frame would be infeasible, even if we had the ships to spare. And even if we were able to deal with all three Forsaken fleets, the Voidbase would still be in a position to launch further attacks.”
The situation is desperate, indeed. An inquisitive shade of yellow rippled down the Hierarch’s scales, I sense you have a request you wish to make, or you would not have demanded an urgent meeting.
Ambassador Evans pursed his lips, “You are correct, Hierarch. Believe me when I say that this is not an easy request for me to make.” He straightened himself to his full height, “As Hierarch, you have command of the Drikenyl Warships that are orbiting Earth, correct?”
For now. The structure of leadership is undergoing some discussion. In times past, it was necessary to separate the Civilian Senate from the Military Hierarchy, to maximize the efficiency of our war efforts while managing a large population base spread out amongst hundreds of stars. With neither a large nor widespread population, coupled with a diminished military force, there may be governmental reformation in the coming months.
“I see.” Ambassador Evans paused, before taking a deep breath, “But before that happens, I’d like to make a formal request for military support from the Drikenyl Military Hierarchy. We need your warships to repel the Forsaken invasion.” A heavy sigh escaped him, but he continued, “I’m reluctant to ask for your soldiers to go off and fight the Forsaken so soon after arriving on Earth, but there are so many innocent Onathins that are dying right now, and many more lives will be lost if we don’t do more. I’ve witnessed the power of your warships in several memory caches, and they would certainly even the odds in our encounters with the Forsaken.”
I sense the battle between your compassion and pragmatism, and I appreciate the fierce struggle against the Forsaken. Subtle shades of blue and yellow danced through the Hierarch’s scales. But we are so few. We need to rebuild our forces while the Onathins fight the Forsaken, just as the Onathins had amassed their forces while we resisted the Forsaken. The Hierarch curled its long body towards the glass, But we will contribute to the war by sharing our defensive technologies. I have been informed that our technicians are already working closely with your scientists, aiding their understanding of Shield Fluidics.
“With limited success.” Ambassador Evans remarked grimly, “Unfortunately, none of the scientists and engineers who are working on the shield projects can converse with the same depth and detail as you and I. The lead physicist has informed me that it would take at least another few years before we can even begin to think about building our own shield cores. We need this technology now.”
Then we shall disconnect the shield cores from our warships. We will install them all across your Homeworld and create an impenetrable planetary barrier.
Ambassador Evans paused again as he chose his next words carefully. He thumbed on his tablet again and watched the casualty numbers from the ongoing battle at Orkina swell onto the screen. “Doing that will definitely protect both of our species. But it would be the wrong course of action.” He flicked a finger across his tablet and threw the scrolling casualty report onto the window-wall between them, “While we hide behind the energy shield, our friends are being slaughtered by the Forsaken. We have to do more. We should always do all that we can to help.”
I have offered the shield cores of my warship fleet. We cannot engage a Forsaken Voidbase without our defenses, nor can our fourty-seven warships fend off an armada of ten thousand vessels. The Hierarch twitched its scales restlessly, What specific task would you ask of us? What is the extent of support that you require?
“You wouldn’t be engaging the Voidbase.” Ambassador Evans stared into the Hierarch’s eyes once more while words and phrases assembled and disassembled themselves in his mind. He flicked his fingers along his tablet again, this time sending a massive schematic onto the window-wall. Tyler pointed at the blueprints of the nearly-completed capital ship, “This is what we are building just outside of the Forge. It is a massive capital ship, one that we think might even be able to destroy a Forsaken Voidbase, provided that it is equipped with certain technologies.”
You wish to install our shield cores onto your new vessel. The Drikenyl wriggled closer to the schematics, studying them curiously while absently picking at a broken scale. Whiskers traced the large, sweeping rings of the human capital ship, while a wingfin brushed over the central spherical core that was protectively surrounded by a pair of hemispherical shells. Your design is…adequate. This can be done, with some modifications. But you cannot expect my warships to challenge the Enemy without their shield cores.
“I’m not.” Ambassador Evans smiled, “I’ve seen the conglomerate ship that carried your civilians to Earth. Every passenger transport within that conglomerate ship carries its own shield core. We can install those onto our capital ship instead.”
But that would leave our people unprotected. A timid shade of orange cascaded throughout the Hierarch’s scales. If there were to be an attack, we would require those shield cores to protect my people.
“Who would attack us?” Ambassador Evans asked, disguising his incredulous tone into the inflection of a genuine question, “The Kredith are bottled up in their last star system, and the Onathins are facing a civil war and the Forsaken incursion simultaneously. Neither humans nor Drikenyl would do anything to attack our common homeworld. There is nothing left to fear except the threat that the Forsaken pose, a threat that will surely eat its way across the Onathin Sovereignty if we don’t do more to oppose it.”
Subtle shades of blue shimmered along the Hierarch’s scales, slowly replacing the orange. It narrowed its bottom eyes and studied Ambassador Evans closely, as if staring directly into his mind, What drives you to help others so fervently?
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MCAT mnemonics

Wanted to share my list of mnemonics that I found helpful for the MCAT. Many of these are from SDN but some are my own. I apologize in advanced for the crude and offensive ones but this often helps recall.
If there's any mnemonics you use for MCAT topics not on here please share!
 

Beta vs. alpha carbohydrates

alpha = trans/down, beta = cis/up bow down to alpha, beta beat me up  

Sympathetic vs. parasympathetic

Sympathetic = fight or flight, parasympathetic = rest & digest Paraplegics do a lot of sitting & resting (this is bad I'm really sorry but it works)  

Electronegativity trend

FONCLBRISCH F > O > N > Cl > Br > I > S > C > H  

Diatomic elements

BrINClHOF  

Water soluble compounds

NAG SHAm Nitrates (NO3-) Acetate (CH3CO2-) Group 1 metal cations (Li, Na, K, Cs, Rb) Sulfates (SO42-) except with PMS Castro Bar Halogens except with PMS Ammonium (NH4+) PMS = Pb (Lead), Mercury (Hg), Silver (Ag) Castro Bar = Ca (Calcium), Str (Strontium), Ba (Barium)  

Acid classifications

Increasing nuance/complexity when in alphabetical order. Arrenhius = acid forms H3O+ & base forms OH- in water (most elementary definition). Brownsted-Lowry = acids donate protons (H+) and bases accept them. Lewis = acids accept electron pairs and bases donate them.  

La Chatelier's principle and equilibrium

When K & Q are in alphabetical order the arrow points to the direction the reaction will go to re-establish equilibrium. K > Q then reaction will go right, increasing product concentration. K < Q then reaction will go left, increasing reactant concentration.  

Anterior pituitary hormones

FLAT PEG Follicle-stimulating (FSH) Luteinizing (LH) Adrenocorticotropic (ACTH) Thyroid stimulating (TSH) Prolactin Endorphins Growth hormone (GH)  

Lysogenic vs. lytic virus lifecycle

Viral DNA incorporated into host genome and just replicated not transcribed (dormant) Lysogenic is a longer word so it takes longer (dormant)  

Retrovirus

retrovirus has RNA reverse transcriptase Two r's in retrovirus: RNA reverse (transcriptase)  

Nerve fibers & location in spinal cord

Sensory afferent, motor efferent, dorsal (towards back) afferent, ventral (towards front) efferent SAME DAVE  

Period of a pendulum

T = 2π√(L/g) pendulum on her period sits on a toliet (L) and g comes out like circumference of a circle (2π*r)  

Calcium hormones

Calcitonin: calcium into bones Parathyroid: rids bone of calcium  

Pyramidines

cytosine, thymine (RNA equivalent uracil) Have y's in them  

Deviations from Hardy-Weinberg Equilibrium

Mutations, migration, drift, non-random mating, selection Maggie May Does Not Smoke  

Cell cycle

Growth phase 1, S phase (DNA synthesis/replication), Growth phase 2, Mitosis, Cytokinesis Go Sally Go Make Children!  

Cell division

Prophase, Metaphase, Anaphase, Telophase PMAT  

Chordate traits

Dorsal nerve cord, notochord, pharyngeal slits, postanal tail Do Not Pinch People  

Embryogenesis

Morula, Blastula, Gastrula Must Be Good  

Fat soluble vitamins

Vitamins D, E, K, A DEKA  

Hormones that increase blood glucose

Somatotropin (growth hormone), thyroid hormones (T3 & T4), epinephrine, norepinephrine, glucagon, glucocorticosteroids, immunoglobulins STENGGI ferengi like sweet blood  

Path of sperm

Seminiferous tubules, epididymis, vas deferens, ejaculatory duct, urethra, penis SEVEN UP  

mRNA post-processing

Exons Expressed, Introns in the trash  

Taxonomy

Kingdom Phylum Class Order Family Genus Species King Philip Came Over For Gold & Silver  

White blood cells

Neutrophils, lymphocytes, monocytes, eosinophils, basophils Nobody Likes Me Eating Badgers  

Valence electrons

H likes to gain 1 e- to be stable, O 2, N 3, C 4 HONC 1234  

Ecell site of redox

Reduction at cathode, oxidation at anode REDCAT ANOX  

State functions

volume (V), Gibb's free energy (G), pressure (P), enthalphy (H), internal energy (E), entropy (S), temperature (T), potential energy (U), density (D) VG PHESTUD  

Basic amino acids

histidine, arginine, lysine HAL  

Cardiac cycle

systole = contraction, diastole = relaxation I Contracted a Cyst  

Diverging lens image

diminished, upright, virtual DDUV  

Acidic cations

Al, Fe, NH4, Zn, Cu, Be, Cr A Fact No Zebra Could Be Creepy  

DNA order

DNA is read 3'-5' left to right like we read. But is synthesized 5'-3' largest to smallest just how a pyramid is built.  

Bacterial conjugation

sexual reproduction in bacteria like a conjugal visit  

Pancreas hormones

insulin, glucagon, somastatin I GLUed ON SOMe TATs to my pancreas
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Skin Basics 1.6.1 - The Acid Mantle - Sweat and Sebum Skin Basics 1.6.1 - The Acid Mantle - Sweat and Sebum

Previous Start Here Reddit’s Too Ugly Follow Along Next
1.5: The pH Scale The Syllabus The Reader App The Email Sign Up 1.6.2: The Acid Mantle
 
Disclaimer
I am not a doctor! Please don’t sue me, I’m already poor!
 

Lesson 1.6.1: The Acid Mantle

 
Welcome back, lovelies! Today, I will finally be delivering on the topic I have been unintentionally postponing for the past who-knows-how-long:
 
*: ͓ °✧ the acid mantle ✧° ͓ : *
 
But did you notice that pesky “.1” stuck in the title? You guessed it -- this subject is getting at least one more post all to itself.
So for now, we’ll be focusing our attention on the substances responsible for making the acid mantle, and the glands responsible for making these substances!
 
Prerequisites:
 

What Is The Acid Mantle?

 
The surface of your skin is acidic, and it has something to do with sweat. That was about as much as we knew on this topic back in 1892, when it was first brought up in a published paper.
Luckily, along came 1928, when German dermatologist Alfred Marchionini and his teacher, H. Shade, published a paper that gave us a better description of this thing they called the “säuremantel”, an acidic film on the surface of the skin that discouraged the growth of bacteria.
With this slightly newer paper unveiling the potential relationship between skin acidity and your overall health, it paved the way for scientists to give more of a crap and start studying it a bit more closely.
We now lovingly refer to this film as the acid mantle.
 
When you are first born, the pH of your acid mantle isn’t all that acidic, measuring in at about 6.4. It’s not until your third or fourth day of existence that the pH will drop down to about 4.9.
Now that you’re older, it probably still has an acidic pH, measuring somewhere between 4.0 and 5.9. That low pH is what gives this film a starring role in your innate immune system, as it is your body’s first line of defense against pathogens.
 
But while acting as your skin’s bacterial bouncer might be the role your acid mantle is most famous for, don’t start typecasting this guy -- your acid mantle has a fantastic resume:
  • It works as a barrier, being one of the many tools your skin uses to prevent water from escaping (because we all know that skin likes to be a moisture hog).
  • It plays a heavy supporting role in making sure your lipid barrier is in tip top shape.
  • And it helps to protect you against the damage caused by free radicals.
 
In today’s lesson, we’ll just be focusing on the junk that forms the acid mantle. But keep these jobs in mind because next time, we will be learning about how your mantle actually performs all of these tasks.
 

Eccrine Sweat Glands

 
It feels like we’ve come a long way since the last time we talked about integumentary accessory structures. Aw, I should start scrapbooking about how far we’ve come. ♡
But now that three of these structures have finally landed a starring role in our lesson, it’s about time we give them more than just a couple of wimpy little paragraphs, don’t you think?
 
As you might remember, your sweat glands come in two flavors: eccrine and apocrine.
Eccrine glands are the type you are most familiar with, as they are the type that adorn your face and the majority of your body. These are also the ones I mentioned in our first lesson that look like knotted spaghetti noodles in your dermis that reach up to the top of your epidermis.
 
Fig. 1, Eccrine Gland Drawing
 
Fig. 2, Eccrine Gland Microscope Slide
 
The knotted portion is called the secretory coil, and is actually a cul-de-sac -- it doesn’t have an input, just an output. The input is actually handled by the cells that line the inside of the coil, which secrete the sweat into the gland. Some of the cells here can contract as well, which is what pushes the perspiration out of the noodle instead of letting it just...sit there.
The noodly portion is known as the sweat duct. The cells here will reabsorb some of the electrolytes in the sweat that had previously been secreted into the coil, leaving your sweat just slightly less salty than it would've been.
The purpose of sweating from these glands is to help regulate body temperature. When you sweat, the cool substance sits on your warm skin’s surface, which helps it to cool down.
The sweat that comes from these glands has a pH between 4.0 and 4.5, and is near 99% water, with the remaining 1% being a cocktail of urea, lactate, sodium, chloride, and potassium.
 

Urea

Urea might sound similar to another word you know...urine! That’s because urea is the byproduct of cellular waste, so the body needs to get rid of it by peeing or sweating it out.
Its chemical formula is CH₄N₂O, which means it has two NH₂ groups joined by a carbonyl group (which is just a carbon that’s double-bonded to an oxygen), like this:
 
Fig. 3, Urea
You don’t see a C for carbon in this picture because it’s assumed that the pointy part of the V shape that’s connecting the two N’s is the carbon. The = connecting that point to the O is the double-bond.
 
Maybe NH₂ sounds a little familiar as well. Perhaps you’re thinking of NH₃ from our acid and base lesson, which is the chemical formula for ammonia. Well, funnily enough, urea is made when your liver breaks down ammonia!
Ammonia is produced by the cellular breakdown of amino acids, but as you know, ammonia is kind of a dangerous chemical. So your liver handles all this junk by consuming two ammonia molecules and one carbon dioxide (CO₂) molecule, then converting them into urea. Makes sense when you see urea happens to contain all of the elements that make NH₃ and CO₂, doesn’t it?
 

Lactate

No, it has nothing to do with nursing a baby. Lactate production is the result exercising!
So, you breathe, right? Cool, me too! Breathing is your body’s preferred way of creating energy, because the oxygen you’re bringing in is super convenient for your cells to access. When your cells use oxygen to create energy, it is called aerobic respiration.
 
Fun Fact: This is where aerobic exercise gets its name from! Aerobic exercises, like walking, jogging, swimming, and cycling, get you breathing more. This heightened level of oxygen input is being used by your cells to quickly produce the energy they need to keep up with the workout, so you can continue to walk, jog, swim, or cycle for prolonged periods of time.
 
But when you engage in a much more strenuous activity, like when you decide to pump some iron instead of hitting the treadmill, your cells end up needing to produce energy faster than the rate at which they’re receiving oxygen. When this happens, they’ll substitute the oxygen in their energy recipe for a different ingredient -- glucose (sugar). When your cells use something other than oxygen to produce energy, it’s known as anaerobic respiration. (And strength training is a type of anaerobic exercise. While you might be able to jog for an hour, you certainly couldn’t do bicep curls for an hour.)
The glucose is broken down by your cells into something called pyruvate. The pyruvate is then converted into lactate, and that lactate allows your cells to continue breaking down glucose while you’re still exercising.
 
By the way, if you are a brethren of /swoleacceptance, you might come across the occasional fitness article using lactic acid interchangeably with lactate. (And if you aren’t praising Brodin, you might recognize it as an AHA!)
So let me clarify that lactate is not the same thing as lactic acid. Your body does not make lactic acid, it makes lactate.
They are definitely related, though! Lactate is the conjugate base of lactic acid, and if you were paying attention during lesson 1.4, you’ll know that this means lactate is the chemical result of lactic acid that has dissociated and lost an H+. So go ahead and slap someone with knowledge the next time you visit the bodybuilders forum! Don’t do this in person, though. I wouldn’t want you to be on the receiving end of some roid rage.
 

Sodium, Chloride, and Potassium

Why did I lump these three together? Because they are all electrolytes. Electrolytes are named such because they are minerals that are either positive or negative ions, giving them an electrical charge.
Sodium regulates the amount of water in your body, and it is required to help generate the electrical signals that allow your various bodily processes to talk to each other (like when your nervous system is talking to your brain about how it didn’t like when you touched the stove). Potassium regulates heartbeat and muscle function -- too much or too little potassium can result in an irregular heartbeat, which can be fatal. Chloride maintains the balance of body fluids.
Electrolytes are clearly all very important, which is why Gatorade is so good at making you feel better after you’ve run a mile and were sweating out your electrolytes, or you’ve been sick and were vomiting up all your electrolytes.
 

Apocrine Sweat Glands

 
I haven’t given these glands very many words before because they aren’t found on your face, preferring only to hang out in all the spots that get hairy during middle school -- in your armpits and in your downstairs mixup. However, since your acid mantle is kind of all over your body, I feel like you guys deserve to know how these glands might affect it.
While these glands also look like spaghetti noodles, they’re a bit larger (so maybe more like worms?), and their noodly portion leads to a hair follicle rather than the skin’s surface. The apocrine sweat empties into the follicle, where it then empties out onto the surface.
 
Fig. 4, Apocrine Sweat Gland
 
(Couldn’t find a satisfactory microscope slide. Sorry!)
 
These glands use the same structural names as their cousins do -- the knotted bit is the secretory coil and the noodly bit is the sweat duct.
 
Fun Fact: While the apocrine glands we’re discussing today are only found in the hairy forests of your body’s landscape, some special, modified versions of these glands than can be found in other areas. There’s some in your nipples that can feed your babies, some in your eyelids that help with killing off bacteria, and some in your ears that make ear wax!
 
The sweat from these glands isn’t very effective at regulating your body temperature because the chemical composition is a little more lipid-y than eccrine sweat (and since these glands aren’t on your face, I won’t bother with a chemical breakdown here).
This sweat also as a more neutral pH, between 6 and 7.5, meaning your mantle isn’t very acidy in your pits. Between the lipidiness and the neutral pH, this sweat will forever leave you in dire need of some deodorant.
Sweat does not stink.
But bacteria, much like humans, enjoy munching on fat way more than they like munching on salty, pee-flavored water. They also thrive in more basic environments. The stink comes from the bacteria tootin’ up a storm while feasting on your armpit buffet. Man, I am just full of beautiful imagery today.
 
If your apocrine sweat doesn’t do any thermoregulating, then why are you cursed with these glands?!
Well, these glands are actually a throwback to your ancient ancestors, with the sweat functioning as a territorial marker (if it smells like you, it’s probably yours), as a warning signal (humans don’t get eaten when they smell gross, I guess), and as a pheromone (only baby-making humans smell like that ;D).
Due to the type of jobs that this sweat is meant for, it makes sense that these glands are activated by hormones rather than temperature. This means they don’t end up getting used until you’ve hit puberty and nature has deemed you old enough to need this kind of sweat. And because they are hormone-activated, this also means that these are the glands that are sweating whenever you are emotionally distraught. (Thanks, guys, for making the pits of my t-shirt wet during all of my high school book report presentations. You really know how to help out.)
 

Sebaceous Glands

 
You should know these guys pretty well by now. Not only have we discussed them before, but they are usually blamed as the source of your skincare woes. To be fair...they often deserve the blame. But with sebum carrying a pH between 4.5 and 5.5, these guys are often under-appreciated for their efforts in the fight against baddies.
 
Fig. 5, Sebaceous Gland
 
Fig. 6, Sebaceous Gland Microscope Slide
 
You might have also noticed in Fig. 6 that the follicle shown has two glands, but it's common for follicles to only have one gland hanging out nearby. They like being attached because, just like apocrine glands, they can deposit their sebum into the follicle, where it then flows out of the pore and onto the skin. The whole enchilada of a follicle paired with a sebaceous gland is known as a pilosebaceous unit.
 
Fun Fact: Some sebaceous glands do open up directly to the skin’s surface. These can be found as little bumps inside your cheeks, on your areolae, and near or on your nono-zone! (I would include pictures, but they’re all NSFW. D:)
 
The gland itself is an outgrowth of the follicle’s sheath, and it is filled with a special little cell called a sebocyte, which is the guy who actually makes the sebum.
You know by now how most cells go about delivering their products, right? They usually make a protein baby, and then poop it out of the cell membrane.
Glands with cells that deliver their junk in this manner are known as merocrine glands, and your eccrine sweat glands are an example of this type.
 
Fig. 7, Merocrine Gland Secretion
 
Some glands produce stuff that’s a little too thick to just poop out; you can think of them as needing some Metamucil. In order to deliver their junk, their cells need to wrap the secretion up in some of their cytosol and cell membrane before just severing the whole package. It sounds painful, but they manage to recuperate just fine.
Glands that specialize in this type of delivery are known as apocrine glands, and unsurprisingly enough, a great example of this type would be your apocrine sweat glands.
 
Fig. 8, Apocrine Gland Secretion
 
Well, our little sebocyte here likes to do things a bit differently. His junk is a lot thicker than the stuff other cells are making; thick enough that it makes him crazy constipated. In fact, he is so constipated that he will end up exploding in the process of trying to poop it all out. Gasp!
Glands whose cells will end up exploding in order to deliver junk are known as holocrine glands. Sebaceous glands are the only holocrine glands found on the human body.
 
Fig. 9, Holocrine Gland Secretion
 
Now that you know sebum is thick enough to make a cell explode, you might be wondering wtf is in this crap. Well, it’s even more lipidy than your apocrine sweat. In fact, its composition is 100% lipid, made up of triglycerides, free fatty acids, wax esters, squalene, cholesterol, and cholesterol esters.
 

Triglycerides

You might recognize this guy from our lesson on the hypodermis. This is the type of lipid that gets stored in your lipocytes, and the stuff you’re trying to get rid of when you start a new diet. This stuff composes the majority of your sebum, making up around 30-50% of it.
The official definition: A triglyceride is an ester of fatty acids and glycerol. In fact, they are sometimes referred to as esters of glycerol.
 
Okay, but what does this mean?
From “tri”, we can guess there is a triplet of some sort. This triplet just so happens to be three chains of fatty acids (more on these in the next section). Any fatty acid will do; some triglycerides have three of a kind, and some could have three totally different fatty acids.
The “glyceride” tells us that these fatty acids are all held together by a glycerol compound (also known as glycerin -- sound familiar?).
 
Fig. 10, Triglyceride Chemical Structure
 
In this picture, the red portion is our glycerol, and the black chains are our three fatty acids. The C’s in those chains are for carbon, and the lines drawn between them actually represent hydrogen molecules that are binding them together. (If you see a zig-zag without C’s, each point of the chain will almost always represent carbon, kind of like back in Fig. 3.)
So what exactly is an ester? It’s an organic compound (meaning, any chemical compound that contains carbon) made by replacing the hydrogen of an acid with a hydrocarbon group (a compound of hydrogen and carbon).
In triglycerides, our hydrocarbon group comes from the glycerol.
 
Fig. 11, Glycerol
 
Now, let’s attach our fatty acids to form an ester. We’ll use stearic acid (CH₃(CH₂)₁₆COOH) as all three of our fatty acids.
 
Fig. 12, Glyceryl Tristearate
 
Oh my...did you notice our stearic acids don’t end in -COOH like I had just said? They end in -COOCH! heh. This is because the hydrogen of our acids were replaced by the glycerol’s hydrocarbon group, a CH. By combining glycerol and three stearic acids, we’ve made glyceryl tristearate, a triglyceride!
 

Free Fatty Acids

Free fatty acids are exactly the same as the fatty acids we discussed above. They’re called “free” because these fatty acids aren’t being used to make any triglycerides, so they are unattached. They make up 15-30% of your sebum.
A fatty acid is an organic compound that contains a carboxyl group (that -COOH we mentioned earlier) and has a long chain of carbons and hydrogens trailing behind it, and the length of that chain can vary anywhere from 10 to 30 carbons (stearic acid had 18, by the way!).
Most of the fatty acids in your sebum tend to be unsaturated. This means that, somewhere along their chain, there is a spot where a carbon atom is bound directly to the next carbon in the chain, instead of every carbon being held together by hydrogen. To help you remember this, think of saturated fats as having a chain that’s fully saturated with hydrogen atoms.
 
Fig. 13, Saturated vs. Unsaturated
 
Saturated fats are solid at room temperature (e.g. butter), whereas unsaturated fats are liquid at room temperature (e.g. oil). This is why your sebum is oily rather than little beads of butter popping out of your pores. This also means you won’t be finding the saturated stearic acid in your sebum, but you will find sapienic acid, sebaleic acid, and linoleic acid, among others.
 
Sapienic acid is the predominant fatty acid in sebum. It gets its name from homo sapiens, because it is really difficult to find this fatty acid anywhere else in nature other than in human sebum. Some studies suggest that this stuff is the big hitter in killing off the bacteria responsible for acne, yet other studies suggest that people with acne have higher levels of sapienic acid than those without acne. In other words, science has yet to figure out what this crap actually does.
Sebaleic acid is pretty similar to sapienic acid, just with two extra carbons. It’s also similar in that it is only found in human sebum, and that it is depressingly understudied. :(
Linoleic acid is used by sebocytes to produce squalene, and the sebum of acne sufferers tends to have lower levels of linoleic acid and higher levels of squalene present. But we’ll get to that more in a minute. (Fun Fact: Linoleic acid isn’t naturally produced by the body, so it needs to come from your diet! You can find it in olive oil.)
 
The exact amount of each fatty acid, free or otherwise, that can be found in sebum will differ from person to person. But it’s worth noting that, while an acne-sufferer tends to produce more sebum than an acne-free person, if you were to take a sample the same amount of sebum from both types of people, the person with acne would actually have 53% fewer free fatty acids of any kind present.
 

Wax Esters

Wax esters make up 26-30% of your sebum. While not unique to humans, they are unique to sebum, as they aren’t produced anywhere else in the body.
Heyyy, weren’t we just talking about esters?! Why, yes!
So we know that triglycerides are an ester of fatty acids and glycerol. Well, wax esters are an ester of a fatty acid and a long-chain alcohol, with the alcohol’s chain being anywhere from 12 to 32 carbons long. Knowing how long fatty acids are, you can tell wax esters are really long.
 
Fig. 14, Wax Ester
 
Now, glycerol is an alcohol. But it doesn’t have a long chain, so triglycerides aren’t considered to also be wax esters. Also, they are triglycerides -- wax esters only have one fatty acid attached to an alcohol.
 
You might see “wax,” and start thinking of candles, Burt’s Bees, or Vaseline (which is actually a mix of wax and oil, by the way). While not all waxes are esters, they do all sort of function in the same waxy way. They seem to be even more water-resistant than oils and fats, and they are fantastic for lubricating and sealing in moisture. This is exactly what the wax esters in your sebum do -- they waterproof your skin, smooth it out, and try to lock in moisture.
The production of wax esters also seems to be related to helping sebocytes level up to the point where they are ready to do their exploding thing. While research has yet to uncover what exactly this relationship is, it has been shown that sebaceous glands tend to waste away when wax esters aren’t getting made properly. These guys do require a lot more studying in the future. (Isn’t it amazing how much we still don’t know about the human body here in the 21st century?)
 

Squalene

This one makes up 12-20% of your sebum, and it is yet another product that is kinda unique to sebum. (Fun Fact: Squalene gets its name from where it was first discovered -- in the liver of Squalidae, or dogfish sharks.)
 
When your body needs to produce a specific chemical, it’s a multi-stage process, with each stage involving a few steps. Think of it as baking a cake. The first stage requires filling a mixing bowl with dry ingredients. But to complete this stage, it has to go through the steps of measuring out, sifting, and adding the flour, baking powder, and salt.
To make cholesterol, the first stage is to make mevalonate. In order to complete this stage, your body needs to take the steps of measuring out three acetyl-CoA’s, then combining them. Stage two is to make isopentenyl pyrophosphate (don’t worry, you don’t need to remember that). And stage three is to make squalene.
 
Normally, the body uses squalene to carry out the fourth and final stage of producing cholesterol. But for some reason, your sebaceous glands are the only places in your body that prefer to halt production at stage three, leaving your sebum with way more squalene than cholesterol. While research has yet to come up with an answer as to why this happens, all we know is that it does, indeed, happen.
There is a theory, however, that the preference for squalene over cholesterol in sebum might be the evolutionary result of pollution. In other words, human sebaceous glands evolved to start making squalene in order to upgrade their skin protection in the face of an increasingly polluted environment.
Such an interesting theory makes it sound like squalene might do something really cool, right? Well, as it turns out, squalene is pretty cool.
See, when squalene is exposed to UV radiation, it begins to gobble up oxygen as an attempt to protect the skin from receiving the full force of the sun. If that doesn’t sound at all cool, don’t worry; we’ll be getting way more in depth about this in the next lesson.
 
Unfortunately, when squalene eats up oxygen, it results in squalene peroxide.
While squalene itself is not harmful to your skin, squalene peroxide definitely is. Your sebum will attempt to make up for the crappy side effects of squalene peroxide by additionally dumping a bunch of vitamin E onto your skin, but the good intentions don’t always cut it. Expect us to be digging into squalene peroxide a lot more in the acne section.
 

Cholesterol and Cholesterol Esters

Cholesterol and its esters make up only a measly 4.5% of your sebum. You can probably guess that the reason for this is because of the preference for squalene.
While cholesterol in cosmetics is used for its moisturizing properties, I honestly couldn’t find much information on the purpose of cholesterol in sebum. This lack of information leads me to believe this is because such an abnormally tiny amount of cholesterol might be viewed as sort of a byproduct of all the squalene production, rather than an “active ingredient,” so to speak. (But that’s just my take on it, so don’t quote that as a fact!)
 
 
That’s it for today, my dears! This lesson is already, like, five pages longer than I prefer them to be. To be fair, I’m not exactly known for brevity. ;3; I hope you’re ready to get slapped with even more säuremantel knowledge next time; my fingers are already itching to get started!
 
ѧѦ ѧ ︵͡︵ ̢ ̱ ̧̱ι̵̱̊ι̶̨̱ ̶̱ ︵ Ѧѧ ︵͡ ︵ ѧ Ѧ ̵̗̊o̵̖ ︵ ѦѦ ѧ ︵͡︵ ̢ ̱ ̧̱ι̵̱̊ι̶̨̱ ̶̱ ︵ Ѧѧ ︵͡ ︵ ѧ Ѧ ̵̗̊o̵̖ ︵ ѧѦ ѧ
 
Hello, everyone!
I hope you weren’t too disappointed by today’s lesson. I know the wait has been long, and I really wanted to squish all of the acid mantle stuff into one lesson, but my character count has forced me to split this subject in two. I’m worried you’re all getting pretty sick of these super science-heavy lessons, since it might not seem immediately clear how any of this info is relevant to managing a skincare issue.
But hopefully you guys will bear with me and just trust that this will all make sense in the end! I really appreciate all of you for sticking around and reading my walls of science text. ♡ Thank you for reading, and leave any questions below. :)
 
  • Please Note:
    There was a lot of info I left out of the section on lactate, like the fact that triglycerides can also be used in anaerobic respiration, or that lactate has nothing to do with post-workout muscle aches. I also left out a lot of chemistry-related info regarding saturated/unsaturated fats and wax esters.
    I did this because I figured these tangents would take us unnecessarily off topic, but if you would like to know more about rest-of-the-body biology or chemistry, feel free to ask about it in the comments or send me an email if you’re on the mailing list. Because, as much as I would enjoy going more in depth on human body biology in a lesson, that’s not really a topic relevant to this subreddit, haha.
 
Sources:
http://link.springer.com/article/10.1007/BF00412626 http://www.ncbi.nlm.nih.gov/pubmed/12964343?dopt=Abstract http://emjreviews.com/wp-content/uploads/Skin-pH-in-the-Elderly-and-Appropriate-Skin-Care.pdf [PDF] http://www.gastrohep.com/ebooks/rodes/Rodes_2_3_7.pdf [PDF] http://www.scientificamerican.com/article/why-does-lactic-acid-buil/ http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835892/ http://www.ncbi.nlm.nih.gov/pubmed/19944183 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835893/ http://chemwiki.ucdavis.edu/Core/Organic_Chemistry/Esters/Properties_of_Esters http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835908/ http://www.ncbi.nlm.nih.gov/pubmed/2936775 http://www.ncbi.nlm.nih.gov/pubmed/12787115 http://www.ncbi.nlm.nih.gov/pubmed/6481825 http://www.jbc.org/content/97/2/433.full.pdf [PDF]
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India Conjugate Vaccine Market Size, Share, News, Demand, Opportunity during 2018-2023

A recent study on Conjugate Vaccine Market by KD Market Insights provides analysis of various factors that can affect the market share. The titled report " Conjugate Vaccine Market: Market Size, Trends, Share & Forecast 2018-2023 " broadly covers the analysis of key market drivers, challenges and trends and gives a better understanding about the forces that shape competition within the industry.
Conjugate vaccine is a type of vaccine that contains bacterial capsular polysaccharide, attached to a protein to enhance immunogenicity and protects against invasive diseases. India is one of the most prominent markets of conjugate vaccines.India is expected to be the fastest growing market due to the government’s focus on research and development in the life sciences domain. By making Pneumococcal Conjugate Vaccine (PCV) available under the Universal immunization program (UIP), the government of India is ensuring equitable access to those who need them the most – the underprivileged and underserved. Hib is one of the leading causes of bacterial meningitis in India and a major cause of childhood pneumonia. The introduction of LPV in India is a significant milestone and a step forward towards accelerating child survival in India, and progress towards achieving national health goals and the Millennium Development Goal 4.
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Market Segmentation: o Based on disease indication, the market is segmented into pneumococcal, haemophilus influenza type b, diphtheria-tetanus-pertussis, meningococcal and others. o Based on end user, the market is segmented into paediatric and adult.
Key growth factors: India is projected to be the fastest growing market for conjugate vaccines during the forecast period. Rapidly increasing population, rise in consumer awareness, growing disposable income and healthcare expenditure, modernization of healthcare infrastructure, and a growing medical tourism industry will drive the growth of the Indian market.
Threats and key players: o The India conjugate vaccine market is expected to have significantly high growth. Delayed introduction of conjugate vaccines is likely to hold back the conjugate vaccine market. Though the prevalence of chronic diseases is high, limited availability and accessibility of conjugate vaccines in remote areas can become a cause for concern. Complicated manufacturing can also be a major restraint for this market.
o Major conjugate vaccine providers operating in the market are GlaxoSmithKline, Pfizer Inc., Merck, Novartis, Sanofi Pasteur, etc.
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Table of Content
Chapter 1: Executive summary 1.1 Market scope and segmentation 1.2 Key questions answered in this study 1.3 Executive summary - I 1.4 Executive summary - II Chapter 2: Market overview 2.1. Market definitions 2.2. India market overview – by revenue - India historical (2015-2017) market revenue (USD Bn) - India forecasted (2018-2023) market revenue (USD Bn) 2.3. India market overview – by volume - India historical (2015-2017) market volume (Million units) - India forecasted (2018-2023) market volume (Million units) 2.4. India market drivers and challenges 2.4.1. India market drivers 2.4.2. India market challenges 2.5. India market trends 2.6. Value chain
Chapter 3: India major segment overview - by disease indication 3.1. Disease indication: Pneumococcal, Hib - by revenue, by volume, CAGR - Overview - by disease indication - Market share (2015, 2018 & 2023): Pneumococcal – India (USD Bn, Million units) - Market share (2015, 2018 & 2023): Hib – India (USD Bn, Million units) 3.2. Disease indication: DTP, Meningococcal - by revenue, by volume, CAGR - Overview - by disease indication - Market share (2015, 2018 & 2023): DTP – India (USD Bn, Million units) - Market share (2015, 2018 & 2023): Meningococcal – India (USD Bn, Million units)
Chapter 4: India major segment overview - by end user 4.1. End-user: Pediatric, Adult - by revenue, by volume, CAGR - Overview - by end user - Market share (2015, 2018 & 2023): Paediatric – India (USD Bn, Million units) - Market share (2015, 2018 & 2023): Adult – India (USD Bn, Million units)
Chapter 5: Competitive landscape 5.1. Company market share - Top 5 companies 5.2. Company overview - GlaxoSmithKline - Company snapshot - Product offerings - Growth strategies - Initiatives - Geographical presence 5.2(A) Executive profile – GlaxoSmithKline 5.3. Company overview – Pfizer Inc. - Company snapshot - Product offerings - Growth strategies - Initiatives - Geographical presence 5.3(A) Executive profile - Pfizer Inc. 5.4. Company overview – Merck & Co. - Company snapshot - Product offerings - Growth strategies - Initiatives - Geographical presence 5.4(A) Executive profile - Merck & Co. 5.5. Company overview – Novartis
- Company snapshot - Product offerings - Growth strategies - Initiatives - Geographical presence 5.5(A) Executive profile - Novartis 5.6. Company overview – Sanofi Pasteur - Company snapshot - Product offerings - Growth strategies - Initiatives - Geographical presence 5.6(A) Executive profile - Sanofi Pasteur 5.7. Company overview – CSL Limited - Company snapshot - Product offerings - Growth strategies - Initiatives - Geographical presence 5.7(A) Executive profile - CSL Limited 5.8. Company overview – Bharat Biotech - Company snapshot
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bacterial conjugation definition video

Bacterial conjugation - YouTube bacterial conjugation - YouTube Bacterial Conjugation - YouTube DNA Exchange: Transformation, Conjugation & Transduction ... - YouTube

Bacterial Conjugation. Bacterial conjugation is one of the principal means of dissemination of genetic information among microbes and from bacteria to other domains of life including plants and other eukaryotes. From: Encyclopedia of Microbiology (Third Edition), 2009. Related terms: Plasmid; Nested Gene; Bacterium; Conjugation; Mutation; Bacteriophage Bacterial Conjugation: steps and mechanism of transfer of plasmid from donor to recipient cell. Conjugation in bacteria is a process in which plasmids are transferred by themselves alone or along with other DNA element from one cell to another cell through conjugation tube. Conjugation occur by physical contact between cells. Bacterial Conjugation Definition Bacterial conjugation is a way by which a bacterial cell transfers genetic material to another bacterial cell.. Bacterial conjugation Jump to: navigation, search Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. Definition. Bacterial conjugation is the transfer of genetic. information between bacterial cells by a bridge- recognition during bacterial conjugation mediated by. F-like plasmids. Let's recap: Conjugation is a mechanism for the horizontal transfer of genes between bacterial cells. This is the only mechanism of horizontal gene transfer that requires physical contact between... bacterial conjugation A kind of sexual intercourse between bacteria in which, after close contact between them, DNA is transferred from one to the other. Some bacteria have a tubular structure on the external surface known as a sex pilus. This is believed to be the route of transfer of DNA during conjugation. Bacterial conjugation is a way by which a bacterial cell transfers genetic material to another bacterial cell. The genetic material that is transferred through bacterial conjugation is a small plasmid, known as F-plasmid (F for fertility factor), that carries genetic information different from that which is already present in the chromosomes of the bacterial cell. Bacterial conjugation is a genetic transfer that involves cell‐to‐cell between donor and recipient cells. With the current method used to teach students in genetic courses at the undergraduate level, the transconjugants are identified using bacterial physiology and/or antibiotic resistance. Bacterial Conjugation Definition. Conjugation is the transfer of a plasmid or other self-transmissible DNA element and sometimes chromosomal DNA from a donor cell to a recipient cell via direct contact usually mediated by a conjugation pilus or sex pilus. Recipients of the DNA transferred by conjugation are called transconjugants. Bacterial Conjugation (n.) 1. ( MeSH ) A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate PROTOZOA for achieving exchange of chromosome material during fusion of two cells.

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Bacterial conjugation - YouTube

About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features Press Copyright Contact us Creators ... Edited by Dr.Osama Ma3rof Teaching Assistant in the microbiology departmentFaculty of pharmacy Facebook profile : www.facebook.com/Dr.Osama.MaaroufFacebook g... https://Biology-Forums.com Ask questions here: https://Biology-Forums.com/index.php?board=3.0 Facebook: https://facebook.com/StudyForcePS/ Instagram: ht... This video “DNA Exchange: Transformation, Conjugation & Transduction” is part of the Lecturio course “Microbiology” WATCH the complete course on http://lec... Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube.

bacterial conjugation definition

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